Team:TzuChiU Formosa/Project
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The reason why we have chosen to use the IPTG induction is because this inducible system complies with the need of our experiment. After IPTG is added, it will combine with LacI thus detaching from the sequence from the Lac operator. This will then activate transcription to our antisense gene and the transcribed antisense mRNA will bind to resistant gene CamR. If we have successfully knocked down CamR, and it cannot be cultured onto the LB plate, then it proves that our idea has worked! | The reason why we have chosen to use the IPTG induction is because this inducible system complies with the need of our experiment. After IPTG is added, it will combine with LacI thus detaching from the sequence from the Lac operator. This will then activate transcription to our antisense gene and the transcribed antisense mRNA will bind to resistant gene CamR. If we have successfully knocked down CamR, and it cannot be cultured onto the LB plate, then it proves that our idea has worked! | ||
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+ | <font size="4"><b>Our plan</b></font> | ||
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+ | The figure below is the composite we plan to use in our project: | ||
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+ | We intend to make use of the iGEM biobricks and the parts we constructed to compose an IPTG inducible system, via the Biobrik Assembly Kit. The T7 promoter(BBa_I719005)、T7 terminator(BBa_K731721) and Lac promoter(BBa_I14032) are igem biobricks. Joining the biobrick with our Lac operator+CamR antisense (BBa_K1222984, BBa_K1222983, BBa_K1222982, BBa_K1222981) and LacI (BBa_K1222003) would be the IPTG inducible system part (BBa_K1222000) we are going to submit. | ||
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+ | The figure below is the biobrick assemble kit overview: | ||
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+ | <img src=""> | ||
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Revision as of 16:29, 27 September 2013