Collaboration

All of the work that was done during this project was both planned and executed by our iGEM team members. It all started of with some brainstorming sessions that began in the middle of May, although the actual lab work began in the beginning of June.

During the summer we have worked very closely with the Linköpings iGEM team. Since it was their first year to participate in the competition we decided to do our best to provide them with any assistance they needed, such as providing them with parts, knowledge etc. In exchange, they provided us with some very valuable information about basic modeling that we could use when modeling our own system.

In addition to assisting Linköpings iGEM team we have also sent biobricks to the following iGEM teams:

UT-Tokyo iGEM 2013:
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein

iGEM Braunschweig 2013:
BBa_K1033930 - amilCP, blue/purple chromoprotein (incl RBS)
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein

Tsinghua iGEM 2013:
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein
BBa_K1033930 - amilCP, blue/purple chromoprotein (incl RBS)

CU Boulder iGEM 2013:
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein
BBa_K1033930 - amilCP, blue/purple chromoprotein (incl RBS)

uOttawa iGEM 2013
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein

HokkaidoU iGEM 2013
BBa_K592009 - amilCP, blue chromoprotein (incl RBS)
BBa_K1033931 - amilGFP, yellow chromoprotein (incl RBS)
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein

TU Darmstadt iGEM 2013
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K1033929 - aeBlue, blue chromoprotein (incl RBS)
BBa_K1033928 - aeBlue, blue chromoprotein (incl RBS, J23110)
BBa_K1033903 - tsPurple, purple chromoprotein (incl RBS, J23110)
BBa_K1033930 - amilCP, blue/purple chromoprotein (incl RBS)
BBa_K1033931 - amilGFP, yellow chromoprotein (incl RBS)

We would also like to thank other sources that have helped us, such as the Slovenian iGEM team 2010 that suppied us with the genes needed for the production of carotenoid metabolites, TU-Munichs iGEM team 2012 that provided us with the gene 4CL for the production of 4-coumaryl-CoA, last but not least we would also like to thank the School of chemical and Biomolecular Engineering, Cornell University for providing us with the genes involved in the production of the phenylpropanoid pathway. The article can be found at; Conrado RJ, Mori J et al (2011)

In addition to this we would also like to mention some fascinating requests that have been made by external sources:

Nature reviews request to display our chromoproteins on their front page for their 10 year anniversary

National Aeronautics and Space Administrations (NASA) biotechnology department requested our chromoproteins as reporter genes for a project that is currently being worked on.