Team:Uppsala/betacarotene

From 2013.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 19: Line 19:
/*Add favicon*/ $("link[rel='shortcut icon']").remove(); $("head").append("<link rel='shortcut icon' type='image/png' href='https://static.igem.org/mediawiki/2013/6/6d/Uppsalas-cow-con.png'>"); });
/*Add favicon*/ $("link[rel='shortcut icon']").remove(); $("head").append("<link rel='shortcut icon' type='image/png' href='https://static.igem.org/mediawiki/2013/6/6d/Uppsalas-cow-con.png'>"); });
</script>
</script>
 +
 +
<link href="https://2013.igem.org/Team:Uppsala/lightbox-css-code.css?action=raw&ctype=text/css" type="text/css" rel="stylesheet">
 +
 +
<script src="https://2013.igem.org/Team:Uppsala/jquery-code.js?action=raw&ctype=text/javascript" type="text/javascript"></script>
 +
 +
<script src="https://2013.igem.org/Team:Uppsala/lightbox-code.js?action=raw&ctype=text/javascript" type="text/javascript"></script>
</head>
</head>
Line 65: Line 71:
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                     <ul>
                                                     <ul>
-
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">P-coumaric acid</a></li>
+
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">p-Coumaric acid</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
Line 86: Line 92:
<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
<ul>
<ul>
-
<li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">P-Coumaric acid</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">Kinetic model</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li>
 +
 +
<li><a href="https://2013.igem.org/Team:Uppsala/toxicity-model">Toxicity model</a></li>
</ul></li>
</ul></li>
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li>
Line 96: Line 104:
<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li>
 +
                                                <li><a href="https://2013.igem.org/Team:Uppsala/advisors">Advisors</a></li>
</ul></li>
</ul></li>
Line 105: Line 114:
<li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li>
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li>
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li>
-
 
+
<li><a href="https://2013.igem.org/Team:Uppsala/bioart">BioArt</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/LactonutritiousWorld">A LactoWorld</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/killswitches">Killswitches</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/realization">Patent</a></li>
</ul></li>
</ul></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a>
+
<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a></li>  
-
                                <ul>
+
-
                                    <li><a href="https://2013.igem.org/Team:Uppsala/collaboration">Collaboration</a></li>
+
-
                                </ul></li>  
+
<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a>
                                     <ul>
                                     <ul>
                                         <li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li>
                                         <li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li>
 +
                                        <li><a href="https://2013.igem.org/Team:Uppsala/protocols">Protocols</a></li>
                                     </ul></li>
                                     </ul></li>
</ul>
</ul>
Line 128: Line 138:
         <div id="clear"></div>
         <div id="clear"></div>
-
<p>β-carotene is a common carotenoid that derives from the xanthophyll group known for its characteristic orange color. β-carotene can be found in fruits and vegetables, for example carrots. β-carotene is a provitamin to vitamin A, which means that during the right circumstances β-carotene can be catalyzed further to produce vitamin A. Vitamin A has several important health aspects which includes skin, mucus membrane and most common to people a good effect on eyes and vision. Studies have shown that the carotenoid can prevent aging of the brain and stimulate it to keep its capability of memory when a high ascorbic acid concentration also is present <sup><a href="#l1">(1)</a></sup>. The health aspects were an important part of our choice to produce β-carotene.</p>
+
<p>β-carotene is a common carotenoid that derives from the xanthophyll group known for its characteristic orange color. β-carotene can be found in fruits and vegetables, for example carrots. β-carotene is a provitamin to vitamin A, which means that during the right circumstances β-carotene can be catalyzed further to produce vitamin A. Vitamin A has several important health aspects which includes skin, mucus membrane and most common to people a good effect on eyes and vision. Studies have shown that the carotenoid can prevent aging of the brain and stimulate it to keep its capability of memory when a high ascorbic acid concentration also is present <sup><a href="#ref1">[1]</a></sup>. The health aspects were an important part of our choice to produce β-carotene.</p>
-
         <img class="molecule" src="https://static.igem.org/mediawiki/2013/f/f4/Uppsala2013_Beta-carotene-molecule.png">
+
         <a href="https://static.igem.org/mediawiki/2013/f/f4/Uppsala2013_Beta-carotene-molecule.png" data-lightbox="roadtrip"><img class="molecule" src="https://static.igem.org/mediawiki/2013/f/f4/Uppsala2013_Beta-carotene-molecule.png"></a>
<a id="b1"></a><h1>Methods</h1>
<a id="b1"></a><h1>Methods</h1>
<p>The gene CrtY that is responsible for translating the protein lycopene cyclase was obtained from the zeaxanthin operon provided by Slovenia iGEM team 2010. Production of β-carotene is initiated through the catalytic reaction done by the enzyme lycopene cyclase which uses the precursor <a href="https://2013.igem.org/Team:Uppsala/lycopene">lycopene.</a></p>
<p>The gene CrtY that is responsible for translating the protein lycopene cyclase was obtained from the zeaxanthin operon provided by Slovenia iGEM team 2010. Production of β-carotene is initiated through the catalytic reaction done by the enzyme lycopene cyclase which uses the precursor <a href="https://2013.igem.org/Team:Uppsala/lycopene">lycopene.</a></p>
-
         <img class="methods-pic" src="https://static.igem.org/mediawiki/2013/d/d2/Uppsala2013_Beta-caroten-plasmid.png">
+
         <a href="https://static.igem.org/mediawiki/2013/d/d2/Uppsala2013_Beta-caroten-plasmid.png" data-lightbox="roadtrip"><img class="methods-pic" src="https://static.igem.org/mediawiki/2013/d/d2/Uppsala2013_Beta-caroten-plasmid.png"></a>
<h1>Results </h1>
<h1>Results </h1>
-
<p>Beta carotene production was proven through the production of our operon containing <a href="https://2013.igem.org/Team:Uppsala/zeaxantin">zeaxanthin.</a> Since production of zeaxanthin relies on beta carotene as a precursor we could simultaneously prove that we successfully had produced both zeaxanthin and beta carotene through liquid-liquid separation and spectrophotometry <a href="https://2013.igem.org/Team:Uppsala/zeaxantin">(read spectrophotometry of zeaxanthin for more results)</a></p>
+
<p>Beta carotene production was proven through the production of our operon containing <a href="https://2013.igem.org/Team:Uppsala/zeaxanthin">zeaxanthin.</a> Since production of zeaxanthin relies on beta carotene as a precursor we could simultaneously prove that we successfully had produced both zeaxanthin and beta carotene through liquid-liquid separation and spectrophotometry <a href="https://2013.igem.org/Team:Uppsala/zeaxanthin">(read spectrophotometry of zeaxanthin for more results)</a></p>
<!--
<!--
Line 146: Line 156:
-->
-->
 +
 +
<h1>References</h1>
 +
 +
<p><a id="ref1">[1]</a>Perrig WJ, Perrig P, Stähelin HB Institute of Psychology, University of Berne, Switzerland. The relation between antioxidants and memory performance in the old and very old, J Am Geriatr Soc. 1997 Jun;45(6):718-24.</p>
</div> <!-- main_content ends -->  
</div> <!-- main_content ends -->  
Line 151: Line 165:
<div id="bottom-pic">
<div id="bottom-pic">
-
<img class="bottom-pic" src="https://static.igem.org/mediawiki/2013/a/aa/Bottom_picture.png">
+
</div>
</div>

Latest revision as of 15:21, 28 October 2013

β-carotene

β-carotene is a common carotenoid that derives from the xanthophyll group known for its characteristic orange color. β-carotene can be found in fruits and vegetables, for example carrots. β-carotene is a provitamin to vitamin A, which means that during the right circumstances β-carotene can be catalyzed further to produce vitamin A. Vitamin A has several important health aspects which includes skin, mucus membrane and most common to people a good effect on eyes and vision. Studies have shown that the carotenoid can prevent aging of the brain and stimulate it to keep its capability of memory when a high ascorbic acid concentration also is present [1]. The health aspects were an important part of our choice to produce β-carotene.

Methods

The gene CrtY that is responsible for translating the protein lycopene cyclase was obtained from the zeaxanthin operon provided by Slovenia iGEM team 2010. Production of β-carotene is initiated through the catalytic reaction done by the enzyme lycopene cyclase which uses the precursor lycopene.

Results

Beta carotene production was proven through the production of our operon containing zeaxanthin. Since production of zeaxanthin relies on beta carotene as a precursor we could simultaneously prove that we successfully had produced both zeaxanthin and beta carotene through liquid-liquid separation and spectrophotometry (read spectrophotometry of zeaxanthin for more results)

References

[1]Perrig WJ, Perrig P, Stähelin HB Institute of Psychology, University of Berne, Switzerland. The relation between antioxidants and memory performance in the old and very old, J Am Geriatr Soc. 1997 Jun;45(6):718-24.