Team:Bielefeld-Germany/SinceRegionals

From 2013.igem.org



Since Regionals


Overview

Since the European Jamboree in Lyon we have been busy and continued working on our project. We managed to create serveral devices by different combinations of the mediator producing parts and the outer membrane porin, did some modelling on the maximal yield of glycerol as a carbon source and conducted additional measurements with our MFC. We even printed a car that was supposed to be powered by our MFC.


Genetic Optimization


  • We constructed new devices by combination of the mediator producing parts (riboflavin synthesis gene cluster and glycerol dehydrogenase) with the outer membrane porin OprF.

    1. BBa_K1172566 - OprF and GldA (Device): Combination of the outer membrane porin OprF (BBa_K1172502) and the glycerol dehydrogenase GldA (BBa_K1172201) under control of T7 promoter.

    2. BBa_K1172577 - OprF and GldA (Coding): Combination of the outer membrane porin OprF (BBa_K1172501) and the glycerol dehydrogenase GldA (BBa_K1172201).

    3. BBa_K1172588 - OprF and Riboflavin synthesis gene cluster (Device): Combination of the outer membrane porin OprF (BBa_K1172502) and Riboflavin synthesis gene cluster (BBa_K1172303) under control of T7 promoter.

    4. BBa_K1172599 - OprF and Riboflavin synthesis gene cluster (Coding): Combination of the outer membrane porin OprF (BBa_K1172501) and the Riboflavin synthesis gene cluster (BBa_K1172303).


We managed to show the functionality of the device BBa_K1172588 through fluorescent measurement.


Engineering Optimization

  • We have designed a fuel cell that can be used with an Ag/AgCl reference electrode. We used it to gain a better understanding of the reactions taking place in the cathode chamber.

  • To illustrate the application we 3D printed a car, that was supposed to be powered by our MFC.

  • In order to assess the effectiveness of our system in a better way, different efficiencies and ratios were calculated.


Human Practice










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