Team:Bielefeld-Germany

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<div id="leftcol" style="width:; height:; float:left; margin-left:5px; margin-top:-20px; padding-right:;">
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<h1>Ecolectricity -  <i>current</i>ly available</h1>
 +
<h2>Electric energy by genetically modified <i>E. coli</i>
 +
<br>in a self-designed and -constructed Microbial Fuel Cell</h2>
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<h3>Project</h3>
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<img src="https://static.igem.org/mediawiki/2013/4/41/Mfc_setup1_small.jpg" height="200px" style="display: block;">
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<h2>Project</h2>
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<p align="justify" style="font-style:normal;"><i>This year the iGEM team 2013 decided to do research on microbial fuel cells (MFC). This project includes genetical modification of </i>Escherichia coli<i> and engineering of a suitable fuel cell to gain electricity. </i></p>
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Abstract" class="info">Read more</a>
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</div>
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</div>
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<h2>Introduction </h2>
 
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<p>
 
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Over 100 years ago the British botanist M. C. Potter discovered electro-chemical reactions by anaerobe microbial degradation processes. This was the milestone for the first thoughts of developing a system for degradation of substances with additional generating of electricity.
 
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<br><br>
 
-
There is a growing interest in the use of alternative energy sources. Decreasing use of fossil fuels in the context of atmospheric greenhouse gas reduction, coupled with the recent reduction of nuclear power production in Germany have encouraged the search for alternative energy production processes. Generation of electricity at large scales can have negative impacts on local environments and the use of energy storage devices on a small scale holds the risk of release of toxic pollutants from batteries.
In consideration of these issues, the iGEM team of Bielefeld University is undertaking a project called "Microbial fuel cell" (MFC) this year. The goal of this project is to use bacteria for direct energy production from organic substrates.
 
-
<br><br>
 
-
Unlike conventional batteries, a Biobattery is environmentally friendly and easy to “charge developing countries. In contrast to wind and solar energy, electricity production is controlled in the MFC by a targeted substrate supply providing direct energy when it is required and avoiding the need for complex storage systems. Exhibiting rapid growth with the addition of simple substrates, bacteria are available within minimal time and in a large” through substrate refilling. Due to its simple design, the MFC could be used in areas with occasional power shortages as a back up energy generation system, for example in quantity. The iGEM team at Bielefeld wants to test different bacteria strains as electron donors, as well as design our own MFC to improve electron transfer and electricity production. Using BioBrick concept and ''Escherichia coli'' as a model organism, we investigate different genetic approaches in order to ensure efficient power generation from the microbial system.
 
-
In addition to the electrical power production on the anode, we will attempt to use the reduction potential of the MFC-cathode for a bioremediation strategy.
 
-
<br><br>
 
-
There are different fields of application to use a MFC.
 
-
For example the usage of the MFC in a wastewater treatment plant to produce electricity by degradation substances which are in the water. This could be an important move to switch the wastewater from an uninteresting, stinky broth to an interesting energy-gaining and so more effective degradation process. Thereby it would be possible to remove recesses of medicals like ethylestradiol or toxic heavy metals like even uran.
 
-
<br><br>
 
-
Another application of an MFC is the demineralization of seawater. This can be done without or with very low energy input to assemble drinking water out of seawater.
 
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<h3>Team</h3>
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  <small>Created with <a href="http://www.admarket.se" title="Admarket.se">Admarket's</a>  
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<img src="https://static.igem.org/mediawiki/2013/5/5d/Bielefeld-Germany-Team-Teamfoto2013_klein.jpg" height="200px"  
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<h2>Team</h2>
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<p align="justify">Our team consists of ten bachelor and master students who study molecular biotechnology and genome-based systems biology. Get to know us better.</p>
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Team" class="info">Read more</a>
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</div>
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<h3>Biosafety</h3>
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<img src="https://static.igem.org/mediawiki/2013/d/d1/Bielefeld-Germany_Biosafety_frontpage.jpg" height="200px" style="display: block; margin: 0 auto;">
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<div class="mask">
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<h2>Biosafety</h2>
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<p>Biosafety is an essential aspect when you work with living organisms that could possibly escape out of your application via damage or incorrect handling. We designed biosafety systems which counter this risk.</p>
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Biosafety" class="info">Read more</a>
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<div class="view " id="results" >
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<div class="hl"">
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<h3>Results</h3>
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</div>
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<img src="https://static.igem.org/mediawiki/2013/3/3c/IGEM_Bielefeld2013_Results_Orange2_Slide_small.jpg" height="200px" style="display: block;">
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<div class="mask">
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<h2>Results</h2>
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<p>Our aim was to produce electricity with our application, which we finally achieved successfully. On the way we got a lot of data, which we analyzed and formatted into graphics. Take a look.</p>
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Results" class="info">Read more</a>
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</div>
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<div class="hl"">
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<h3>Human Practice</h3>
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</div>
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<div id=help>
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<img src="https://static.igem.org/mediawiki/2013/2/20/Bielefeld-Germany2013-Streetscience1.jpg" height="200px"
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<div class="mask">
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<h2>Human Practice</h2>
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<p align="justify">It is important to get out of the lab and tell the public about your project and about synthetic biology. Therefore we arranged events and visited conferences in order to get in touch with the public.</p>
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/HumanPractice" class="info">Read more</a>
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<div class="view " id="labjournal" >
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<h3>Labjournal</h3>
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<img src="https://static.igem.org/mediawiki/2013/1/10/Bielefeld-Germany-Labjournal_Foto1.jpg" height="200px"
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<div class="mask">
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<h2>Labjournal</h2>
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<p>We wrote all the experiments we did into a labjournal. We digitalized the lab journal so that everyone can comprehend our experiments and achieve reproducible results.</p>
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Labjournal" class="info">Read more</a>
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<h3>Pictures & Videos</h3>
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</div>
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<div id=help>
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<img src="https://static.igem.org/mediawiki/2013/f/f5/Bielefeld-Germany2013-yt_printing.jpg" height="200px"
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<h2>Pictures & Videos</h2>
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<p>During the work on our project we took a lot of nice pictures and recorded some videos inside and outside the lab. See a collection of our favorites on our media page.</p>
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Media" class="info">Read more</a>
 +
</div>
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</div>
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<div class="view " id="modelling" >
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<div class="hl"">
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<h3>Modelling</h3>
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</div>
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<div id=help>
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<img src="https://static.igem.org/mediawiki/2013/5/52/Bielefeld-Germany-Modelling_overview.jpg" height="200px"
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style="display: block;">
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<div class="mask">
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<h2>Modelling</h2>
 +
<p>To predict various processes in our MFC we did some modelling on different reactions and parameters.</p>
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Modeling" class="info">Read more</a>
 +
</div>
 +
</div>
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<div class="view " id="regionals" >
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<div class="hl"">
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<h3>Since Regionals</h3>
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<div id=help>
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<img src="https://static.igem.org/mediawiki/2013/c/c2/Bielefeld-Germany_SinceRegionals.jpg" height="200px"
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<div class="mask">
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<h2>Since Regionals</h2>
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<p>After the European Jamboree we were busy and continued working on our project to optimize it further and conduct the last measurements.</p>
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/SinceRegionals" class="info">Read more</a>
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<h1>Achievements</h1>
 +
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[[Image:Bielefeld-Germany-Box_checked_orange.png|80px]]<font size="5">First Runner Up Overgraduate</font>
 +
[https://igem.org/Results?year=2013&region=Championship&division=igem (Official results)]
 +
<br>
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|80px]]<font size="5">Best Food & Energy Project Overgraduate</font>
 +
[https://igem.org/Results?year=2013&region=Championship&division=igem (Official results)]
 +
<br>
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|80px]]<font size="5">European Grand Prize [https://static.igem.org/mediawiki/2013/9/9f/Bielefeld-Germany_GrandPrizeWinner.JPG Winner] Overgraduate</font>
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[https://igem.org/Results?year=2013&region=Europe&division=igem (Official results)]
 +
<br>
 +
<br>
 +
<font size="3">
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[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Award for the [https://static.igem.org/mediawiki/2013/7/75/Bielefeld-Germany_bestprese.JPG best presentation] europe overgraduate
 +
[https://igem.org/Results?year=2013&region=Europe&division=igem (Official results)]
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Design, construction and development of a [https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC MFC] over three generations
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Design and construction of a biodegradable [https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC#3D_Printing 3D-printed MFC]
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Established measurement parameters for the [https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC MFC] and used it for biobrick characterization
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Electricity generation with the [https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC#Results MFC]
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Construction of [https://2013.igem.org/Team:Bielefeld-Germany/Project/Porins OprF] expressing Biobricks
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Construction of [https://2013.igem.org/Team:Bielefeld-Germany/Project/GldA glycerol dehydrogenase] expressing Biobricks
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Construction of [https://2013.igem.org/Team:Bielefeld-Germany/Project/Riboflavine riboflavin] producing Biobricks
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Improvement of the Biobrick [http://parts.igem.org/Part:BBa_K914014?title=Part:BBa_K914014 BBa_K914014]
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Development and successful verification of three novel [https://2013.igem.org/Team:Bielefeld-Germany/Biosafety/Biosafety_System Biosafety-Systems]
 +
<br>
 +
[[Image:Bielefeld-Germany-Box_checked_orange.png|60px]] Helped another [https://2013.igem.org/Team:Bielefeld-Germany/Collaborations team]
 +
<br>
 +
</font>
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</div>
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[[Image:MFC-Principle.png|377x400px|thumb|center|MFC-Principle|<div style="text-align:justify;">'''Image 1:''' Principle of an microbial fuel cell (MFC). On the anodic side the microorganism is producing electrons and protons by metabolizing the fuel. Then the protons will be transferred via the membrane, while the electrons will be transferred to the anode. When the electrons reduce an electron acceptor on the cathodic side of the MFC, there will be an electrical current flow.  ([[Media:MFC-Principle.png|Zoom in]])</div>]]
 
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Latest revision as of 17:55, 14 October 2014





Ecolectricity - currently available

Electric energy by genetically modified E. coli
in a self-designed and -constructed Microbial Fuel Cell


Project

Project

This year the iGEM team 2013 decided to do research on microbial fuel cells (MFC). This project includes genetical modification of Escherichia coli and engineering of a suitable fuel cell to gain electricity.

Read more

Team

Team

Our team consists of ten bachelor and master students who study molecular biotechnology and genome-based systems biology. Get to know us better.

Read more

Biosafety

Biosafety

Biosafety is an essential aspect when you work with living organisms that could possibly escape out of your application via damage or incorrect handling. We designed biosafety systems which counter this risk.

Read more

Results

Results

Our aim was to produce electricity with our application, which we finally achieved successfully. On the way we got a lot of data, which we analyzed and formatted into graphics. Take a look.

Read more

Human Practice

Human Practice

It is important to get out of the lab and tell the public about your project and about synthetic biology. Therefore we arranged events and visited conferences in order to get in touch with the public.

Read more

Labjournal

Labjournal

We wrote all the experiments we did into a labjournal. We digitalized the lab journal so that everyone can comprehend our experiments and achieve reproducible results.

Read more

Pictures & Videos

Pictures & Videos

During the work on our project we took a lot of nice pictures and recorded some videos inside and outside the lab. See a collection of our favorites on our media page.

Read more

Modelling

Modelling

To predict various processes in our MFC we did some modelling on different reactions and parameters.

Read more

Since Regionals

Since Regionals

After the European Jamboree we were busy and continued working on our project to optimize it further and conduct the last measurements.

Read more

Achievements

Bielefeld-Germany-Box checked orange.pngFirst Runner Up Overgraduate (Official results)

Bielefeld-Germany-Box checked orange.pngBest Food & Energy Project Overgraduate (Official results)

Bielefeld-Germany-Box checked orange.pngEuropean Grand Prize Winner Overgraduate (Official results)

Bielefeld-Germany-Box checked orange.png Award for the best presentation europe overgraduate (Official results)
Bielefeld-Germany-Box checked orange.png Design, construction and development of a MFC over three generations
Bielefeld-Germany-Box checked orange.png Design and construction of a biodegradable 3D-printed MFC
Bielefeld-Germany-Box checked orange.png Established measurement parameters for the MFC and used it for biobrick characterization
Bielefeld-Germany-Box checked orange.png Electricity generation with the MFC
Bielefeld-Germany-Box checked orange.png Construction of OprF expressing Biobricks
Bielefeld-Germany-Box checked orange.png Construction of glycerol dehydrogenase expressing Biobricks
Bielefeld-Germany-Box checked orange.png Construction of riboflavin producing Biobricks
Bielefeld-Germany-Box checked orange.png Improvement of the Biobrick BBa_K914014
Bielefeld-Germany-Box checked orange.png Development and successful verification of three novel Biosafety-Systems
Bielefeld-Germany-Box checked orange.png Helped another team




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