Team:Colombia Uniandes/Parts

From 2013.igem.org

(Difference between revisions)
(Glucocorticoid sensor)
(Glucocorticoid sensor)
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You can see the confirmation gels of the parts below:  
You can see the confirmation gels of the parts below:  
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[[File:Gel1GC.jpg|400px|thumb|left|''' PCR confirmation: E1GF-E1GF(2)-E2GF-E2GF(2)-WM-E3GF-E3GF(2)-E4GF-E4GF(2)''']]  [[File:Gel2GC.jpg|400px|thumb|center|'''Co-transformation: WM-E1GF2-E2GF-E3GF1-E3GF2-E4GF-WM- E3GF3-E4GF2-E2GF2
[[File:Gel1GC.jpg|400px|thumb|left|''' PCR confirmation: E1GF-E1GF(2)-E2GF-E2GF(2)-WM-E3GF-E3GF(2)-E4GF-E4GF(2)''']]  [[File:Gel2GC.jpg|400px|thumb|center|'''Co-transformation: WM-E1GF2-E2GF-E3GF1-E3GF2-E4GF-WM- E3GF3-E4GF2-E2GF2
''']]
''']]
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[[File:GEL3.jpg|400px|thumb|center|'''PCR confirmation of the parts cloned in the pSB1C3 backbone using the specifically designed primers 15 and 31 (see primers)''']]

Revision as of 02:31, 28 September 2013

Parts

Glucocorticoid sensor

Here are the parts sent to the registry:

PartsGC.jpg

You can see the confirmation gels of the parts below:




PCR confirmation: E1GF-E1GF(2)-E2GF-E2GF(2)-WM-E3GF-E3GF(2)-E4GF-E4GF(2)
Co-transformation: WM-E1GF2-E2GF-E3GF1-E3GF2-E4GF-WM- E3GF3-E4GF2-E2GF2
PCR confirmation of the parts cloned in the pSB1C3 backbone using the specifically designed primers 15 and 31 (see primers)


Also, the fluorescence assays for the E*GF parts

Unsaturated curve where we see how the fluorescence increases after the addition of dexamethasone, a glucocorticoid
Saturated curve for fluorescence. We can see the different strengths depending on the number of UAS boxes


Nickel Removal System

Here are the parts sent to the registry:

PartsNi.jpg
Prcna-HoxN and HoxN-RFP