Primers used to construct Nicko and Chimi
| Name
| Sequence
| Identification Number
|
| FW XbaI-BAP2
| CAC CTC TAG AGA GCA TTA ATG CAA GTC GAG AAA GG
| 19
|
| FW XbaI-GAL1
| CAC CTC TAG AGG CTA TAT TGA AGT ACG GAT TAG
| 15
|
| FW Fusion GAL1-KOZAC-mCherry
| GGA AAC TAG ACC CGC CGC CAC CAT GGA GAT GGT GAG CAA GGG CGA GG
| 17
|
| RV Fusion GAL1-KOZAC-mCherry
| CCT CGC CCT TGC TCA CCA TCT CCA TGG TGG CGG CGG GTC TAG TTT CC
| 13
|
| FW Fusion BAP2-KOZAC-GAL4
| CTC AAG TGA TTT TAG AAC CCG CCG CCA CCA TGG AGA TGA AGC TAC TGT CTT C
| 11
|
| RV Fusion BAP2-KOZAC-GAL4
| GAA GAC AGT AGC TTC ATC TCC ATG GTG GCG GCG GGT TCT AAA ATC ACT TGA G
| 3
|
| FW FUSION GAL4-VP16-1
| CAG TTG ACT GTA TCT TCG AGG TCG ACC ATT CAG ATC TCA CGA GCC GCC CCC CCG ACC G
| 1
|
| RV FUSION GAL4-VP16-1
| CGG TCG GGG GGG CGG CTC GTG AGA TCT GAA TGG TCG ACC TCG AAG ATA CAG TCA ACT G
| 5
|
| FW FUSION VP6-GCR
| CCT ACG GCG CTC TGG ATA TGA TCT CTC GAG CCA TTC AGC AAG CCA CTG CAG G
| 9
|
| RV FUSION VP6-GCR
| CCT GCA GTG GCT TGC TGA ATG GCT CGA GAG ATC ATA TCC AGA GCG CCG TAG G
| 6
|
| FW Fusion GCR-yeastTerminador
| GCT TCT GTT TCA TCA AAA ATG AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG
| 35
|
| RV Fusion GCR-yeastTerminador
| CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTC ATT TTT GAT GAA ACA GAA GC
| 34
|
| FW Fusion mCherry-yeastTerminador
| GGA CGA GCT GTA CAA GTA ATA AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG
| 33
|
| RV Fusion mCherry-yeastTerminador
| CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTT ATT ACT TGT ACA GCT CGT CC
| 32
|
| FW Constructo final
| ACC GTC TAG ATT GAC AGC TAG CTC AGT CCT AGG
| 40
|
| FW HoxN
| ATG GAA GCA CTG CCG AAC GAT T
| 42
|
| RV HoxNconColamRfP
| GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG
| 44
|
| FW mRFPconColaHoxN
| GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA
| 41
|
| RV mRFPconXba
| ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC
| 38
|
| RcnR+B1006 RV
| AAA AAC CCC GCC CTG TCA GGG GCG GGG TTT TTT TTT TTA TTT GAT ATA TGA ATC CAG CAC
| 10
|
| FW Prcna
| GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT
| 43
|
| RV Prcna
| TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT
| 39
|
| J23117+B0034+RcnR FW
| GAC AGC TAG CTC AGT CCT AGG GAT TGT GCT AGC AAA GAG GAG AAA ATG TCT CAT ACA ATC
| 23
|
| J23117+B0034+RcnR RV
| GAT TGT ATG AGA CAT TTT CTC CTC TTT GCT AGC ACA ATC CCT AGG ACT GAG CTA GCT GTC
| 21
|
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