Team:Colombia Uniandes/Parts
From 2013.igem.org
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You can see the confirmation gels of the parts below: | You can see the confirmation gels of the parts below: | ||
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- | + | ==='''Characterization of reporters'''=== | |
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+ | To assess the strength of our promoters when induced with Dexamethasone, we performed a fluorometric assay using mCherry as our reporter. | ||
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[[File:Response1hr.jpg|700px|thumb|center|''' Unsaturated curve where we see how the mCherry reporter appears after the addition of 10 uM dexamethasone, a glucocorticoid. Emmision intensity was measured at 607nm after excitation at 586nm''']] | [[File:Response1hr.jpg|700px|thumb|center|''' Unsaturated curve where we see how the mCherry reporter appears after the addition of 10 uM dexamethasone, a glucocorticoid. Emmision intensity was measured at 607nm after excitation at 586nm''']] | ||
[[File:Response3hr.jpg|700px|thumb|center|''' Saturated curve for fluorescence. After three hours of the addition of 10 uM dexamethasone the reporter (mCherry) reaches its highest signal. We can see the different strengths depending on the number of UAS boxes. Emmision intensity was measured at 607nm after excitation at 586nm''']] | [[File:Response3hr.jpg|700px|thumb|center|''' Saturated curve for fluorescence. After three hours of the addition of 10 uM dexamethasone the reporter (mCherry) reaches its highest signal. We can see the different strengths depending on the number of UAS boxes. Emmision intensity was measured at 607nm after excitation at 586nm''']] | ||
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+ | We also decided to visually inspect our induced transformants. Here two of the images taken using a epifluorescent microscopy with a TRITC filter. | ||
Revision as of 03:03, 28 September 2013
Parts
Glucocorticoid sensor
Here are the parts sent to the registry:
Part Name | Registry Number |
E1 | BBa_K1144001 |
E2 | BBa_K1144002 |
E3 | BBa_K1144003 |
E4 | BBa_K1144004 |
E1GF | BBa_K1144005 |
E2GF | BBa_K1144006 |
E3GF | BBa_K1144007 |
E4GF | BBa_K1144008 |
You can see the confirmation gels of the parts below:
Characterization of reporters
To assess the strength of our promoters when induced with Dexamethasone, we performed a fluorometric assay using mCherry as our reporter.
We also decided to visually inspect our induced transformants. Here two of the images taken using a epifluorescent microscopy with a TRITC filter.