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Team:UANL Mty-Mexico/Wetlab
From 2013.igem.org
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<li><a href="#GFP">GFP thermometer</a></li> | <li><a href="#GFP">GFP thermometer</a></li> | ||
<li><a href="#Conclusions">Transcription factor thermometers</a></li> | <li><a href="#Conclusions">Transcription factor thermometers</a></li> | ||
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</ul> | </ul> | ||
<body> | <body> | ||
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<div class="justified"> | <div class="justified"> | ||
| - | <p> | + | <p>We divided our circuit in sub-circuits, or modules. Each module comprises a single function of our system. These modules are:</p> |
| + | <ul> | ||
| + | <li>The RFP switch.- this switch comprises the 37°C thermometer and an RFP reporter right downstream from it.</li> | ||
| + | <li>The GFP switch.- this switch is similar to the RFP one, but has a 32°C thermometer and a GFP reporter.</li> | ||
| + | <li>The LacI-GFP switch.- in this switch, a 37°C thermometer is regulating the expression of a LacI gene, which in turn is regulating the expression of a GFP reporter. This GFP reporter is also under the regulation of a 32°C thermometer. In this way, we expect to see two different states: OFF at temperatures below 32°C; ON when temperature is between 32°C and 37°C; and oFF again when temperature is above 37°C.</li> | ||
| + | <li>The TetR-RFP switch.-</li> | ||
| + | <li>The cI-TetR-RFP switch.-</li> | ||
| + | </ul> | ||
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<div class="row featurette"> | <div class="row featurette"> | ||
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| - | <h2 class="featurette-heading"> | + | <h2 class="featurette-heading">I<span class="text-muted">Source of intense metagenomic diversity</span></h2> |
<p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.</p> | <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.</p> | ||
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{{:Team:UANL_Mty-Mexico/Templates:Footer}} | {{:Team:UANL_Mty-Mexico/Templates:Footer}} | ||
Revision as of 02:18, 24 September 2013
We divided our circuit in sub-circuits, or modules. Each module comprises a single function of our system. These modules are:
- The RFP switch.- this switch comprises the 37°C thermometer and an RFP reporter right downstream from it.
- The GFP switch.- this switch is similar to the RFP one, but has a 32°C thermometer and a GFP reporter.
- The LacI-GFP switch.- in this switch, a 37°C thermometer is regulating the expression of a LacI gene, which in turn is regulating the expression of a GFP reporter. This GFP reporter is also under the regulation of a 32°C thermometer. In this way, we expect to see two different states: OFF at temperatures below 32°C; ON when temperature is between 32°C and 37°C; and oFF again when temperature is above 37°C.
- The TetR-RFP switch.-
- The cI-TetR-RFP switch.-
ISource of intense metagenomic diversity
Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.
Cuatro ciénegas. Source of intense metagenomic diversity
Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.
