Team:UANL Mty-Mexico/Wetlab

We divided our circuit in sub-circuits, or modules. Each module comprises a single function of our system. These modules are:

• The RFP switch.- this switch comprises the 37°C thermometer and an RFP reporter right downstream from it.
• The GFP switch.- this switch is similar to the RFP one, but has a 32°C thermometer and a GFP reporter.
• The LacI-GFP switch.- in this switch, a 37°C thermometer is regulating the expression of a LacI gene, which in turn is regulating the expression of a GFP reporter. This GFP reporter is also under the regulation of a 32°C thermometer. In this way, we expect to see two different states: OFF at temperatures below 32°C; ON when temperature is between 32°C and 37°C; and oFF again when temperature is above 37°C.
• The TetR-RFP switch.- here, an RFP reporter is regulated by a 37°C thermometer and a pTet promoter; this switch also includes a TetR construction.
• The cI-TetR-RFP switch.- this switch is similar to the previous one, but also includes a cassette that expresses a thermolabile version of cI. In this way, the expression of RFP will be ON only at temperatures between 37°C and 42°C and OFF at other temperatures.

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