Glycerol stocks

Reagents and Materials

• Overnight bacterial growth
• Screw Cap Tubes
• Glycerol

Protocol

1. Pipet 0.5mL of glycerol into two 1.5mL screw cap tubes
2. Add 0.5mL of overnight culture to each tube
3. Pipet up and down to gently mix
4. Place one tube in -20°C freezer
5. Place the other tube in -80°C freezer