Team:Macquarie Australia/Protocols/SDS Page


SDS Page

SDS-Page, image by Andra MIhali

1) Resuspend Pelleted cells in 200 uL Milli-Q H2O.

2) Transfer 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer.

3) Using a Hamilton syringe, shear the cells.

4) Centrifuge the preparations for 3 mins @ 13,000 rpm.

5) Load 20 uL of the supernatant in to the gel.

6) Conduct electrophoresis at a constant voltage (200 V) for one hour.