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Team:Macquarie Australia/Protocols/SDS Page
From 2013.igem.org
SDS Page
1) Resuspend Pelleted cells in 200 uL Milli-Q H2O.
2) Transfer 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer.
3) Using a Hamilton syringe, shear the cells.
4) Centrifuge the preparations for 3 mins @ 13,000 rpm.
5) Load 20 uL of the supernatant in to the gel.
6) Conduct electrophoresis at a constant voltage (200 V) for one hour.
