Team:ETH Zurich/Experiments 7

From 2013.igem.org

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<h1><b>Colorimetric response on LB-Agar</b></h1>
<h1><b>Colorimetric response on LB-Agar</b></h1>
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<h1><b>Beta-galactosidase</b> (LacZ)</h1>
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<b>Beta-galactosidase</b>(LacZ)
[[File:XGAL.png|left|300px|thumb|<b>Fig.5: X-Gal on LacZ expressing colony</b>]]
[[File:XGAL.png|left|300px|thumb|<b>Fig.5: X-Gal on LacZ expressing colony</b>]]
[[File:Green_beta_gal_on_t7_strain.JPG|left|300px|thumb|<b>Fig.6: Beta-green-X-gal on LacZ expressing colony</b>]]
[[File:Green_beta_gal_on_t7_strain.JPG|left|300px|thumb|<b>Fig.6: Beta-green-X-gal on LacZ expressing colony</b>]]
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<h1><b>Alkaline phosphatase</b>(PhoA)</h1>
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<b>Alkaline phosphatase</b>(PhoA)
[[File:PNPP_on_phoA_t7_strain.JPG|left|300px|thumb|<b>Fig.2: NPP on PhoA expressing colony</b>]]
[[File:PNPP_on_phoA_t7_strain.JPG|left|300px|thumb|<b>Fig.2: NPP on PhoA expressing colony</b>]]
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<h1><b>Acetyl esterase</b> (Aes)</h1>
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<b>Acetyl esterase</b> (Aes)
[[File:Magenta_butyrate_on_aes_t7_strain.JPG|left|248px|thumb|<b>Fig.1: Magenta butyrate on Aes expressing colony</b>]]
[[File:Magenta_butyrate_on_aes_t7_strain.JPG|left|248px|thumb|<b>Fig.1: Magenta butyrate on Aes expressing colony</b>]]
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<b>Glycoside hydrolase</b> (NagZ)
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<h1><b>Glycoside hydrolase</b> (NagZ)</h1>
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[[File:XGlcNAc_on_nagZ_t7_strain.JPG|left|300px|thumb|<b>Fig.4: X-Glucnac on NagZ expressing colony</b>]]
[[File:XGlcNAc_on_nagZ_t7_strain.JPG|left|300px|thumb|<b>Fig.4: X-Glucnac on NagZ expressing colony</b>]]
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<h1><b>Beta-glucuronidase</b> (GusA)</h1>
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<b>Beta-glucuronidase</b> (GusA)
[[File:Salmon_glcUA_on_gusA_t7_strain.JPG |left|280px|thumb|<b>Fig.3 Magenta glucuronide on GusA expressing colony</b>]]
[[File:Salmon_glcUA_on_gusA_t7_strain.JPG |left|280px|thumb|<b>Fig.3 Magenta glucuronide on GusA expressing colony</b>]]
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Revision as of 08:35, 9 September 2013

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What about those hydrolases ?

We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).

On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.

Colorimetric response in liquid culture


Colorimetric response on LB-Agar



Beta-galactosidase(LacZ)

Fig.5: X-Gal on LacZ expressing colony
Fig.6: Beta-green-X-gal on LacZ expressing colony


Alkaline phosphatase(PhoA)

Fig.2: NPP on PhoA expressing colony


Acetyl esterase (Aes)

Fig.1: Magenta butyrate on Aes expressing colony


Glycoside hydrolase (NagZ)

Fig.4: X-Glucnac on NagZ expressing colony


Beta-glucuronidase (GusA)

Fig.3 Magenta glucuronide on GusA expressing colony




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