Team:ETH Zurich/Experiments 7
From 2013.igem.org
(Difference between revisions)
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<h1><b>Colorimetric response on LB-Agar</b></h1> | <h1><b>Colorimetric response on LB-Agar</b></h1> | ||
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- | <b>Beta-galactosidase</b> | + | <b>Beta-galactosidase(LacZ)</b><br><br><br> |
[[File:XGAL.png|left|300px|thumb|<b>Fig.5: X-Gal on LacZ expressing colony</b>]] | [[File:XGAL.png|left|300px|thumb|<b>Fig.5: X-Gal on LacZ expressing colony</b>]] | ||
[[File:Green_beta_gal_on_t7_strain.JPG|left|300px|thumb|<b>Fig.6: Beta-green-X-gal on LacZ expressing colony</b>]] | [[File:Green_beta_gal_on_t7_strain.JPG|left|300px|thumb|<b>Fig.6: Beta-green-X-gal on LacZ expressing colony</b>]] | ||
<br clear="all"/> | <br clear="all"/> | ||
- | + | <hr> | |
- | <b>Alkaline phosphatase</b> | + | <b>Alkaline phosphatase(PhoA)</b><br><br><br> |
[[File:PNPP_on_phoA_t7_strain.JPG|left|300px|thumb|<b>Fig.2: NPP on PhoA expressing colony</b>]] | [[File:PNPP_on_phoA_t7_strain.JPG|left|300px|thumb|<b>Fig.2: NPP on PhoA expressing colony</b>]] | ||
<br clear="all"/> | <br clear="all"/> | ||
- | + | <hr> | |
- | <b>Acetyl esterase</b> | + | <b>Acetyl esterase(Aes)</b><br><br><br> |
[[File:Magenta_butyrate_on_aes_t7_strain.JPG|left|248px|thumb|<b>Fig.1: Magenta butyrate on Aes expressing colony</b>]] | [[File:Magenta_butyrate_on_aes_t7_strain.JPG|left|248px|thumb|<b>Fig.1: Magenta butyrate on Aes expressing colony</b>]] | ||
<br clear="all"/> | <br clear="all"/> | ||
- | <b>Glycoside hydrolase</b> | + | <hr> |
- | [[File:XGlcNAc_on_nagZ_t7_strain.JPG|left|300px|thumb|<b>Fig.4: X-Glucnac on NagZ expressing colony</b>]] | + | <b>Glycoside hydrolase(NagZ)</b><br><br><br> |
+ | [[File:XGlcNAc_on_nagZ_t7_strain.JPG|left|padding 20px|300px|thumb|<b>Fig.4: X-Glucnac on NagZ expressing colony</b>]] | ||
<br clear="all"/> | <br clear="all"/> | ||
- | + | <hr> | |
- | <b>Beta-glucuronidase</b> | + | <b>Beta-glucuronidase(GusA)</b><br><br><br> |
[[File:Salmon_glcUA_on_gusA_t7_strain.JPG |left|280px|thumb|<b>Fig.3 Magenta glucuronide on GusA expressing colony</b>]] | [[File:Salmon_glcUA_on_gusA_t7_strain.JPG |left|280px|thumb|<b>Fig.3 Magenta glucuronide on GusA expressing colony</b>]] | ||
<br clear="all"/> | <br clear="all"/> | ||
<br><br> | <br><br> |
Revision as of 08:39, 9 September 2013
What about those hydrolases ?
We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).
On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
Beta-galactosidase(LacZ)
Alkaline phosphatase(PhoA)
Acetyl esterase(Aes)
Glycoside hydrolase(NagZ)
Beta-glucuronidase(GusA)