Team:ETH Zurich/Experiments 7

From 2013.igem.org

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<h1>Colorimetric response in liquid culture</h1>
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<h1>Beta-galactosidase(LacZ)</h1>
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<b>Colorimetric response in liquid culture</b>
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<h1>Colorimetric response on LB-Agar</h1>
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<b>Colorimetric response on LB-Agar</b>
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<b>Beta-galactosidase(LacZ)</b><br><br><br>
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[[File:XGAL.png|left|150px|thumb|<b>Fig.5: X-Gal on LacZ expressing colony</b>]]
[[File:XGAL.png|left|150px|thumb|<b>Fig.5: X-Gal on LacZ expressing colony</b>]]
[[File:Green_beta_gal_on_t7_strain.JPG|left|150px|thumb|<b>Fig.6: Beta-green-X-gal on LacZ expressing colony</b>]]
[[File:Green_beta_gal_on_t7_strain.JPG|left|150px|thumb|<b>Fig.6: Beta-green-X-gal on LacZ expressing colony</b>]]
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<b>Alkaline phosphatase(PhoA)</b><br><br><br>
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<h1>Alkaline phosphatase(PhoA)</h1>
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<b>Colorimetric response in liquid culture</b>
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<b>Colorimetric response on LB-Agar</b>
[[File:PNPP_on_phoA_t7_strain.JPG|left|150px|thumb|<b>Fig.2: NPP on PhoA expressing colony</b>]]
[[File:PNPP_on_phoA_t7_strain.JPG|left|150px|thumb|<b>Fig.2: NPP on PhoA expressing colony</b>]]
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<b>Acetyl esterase(Aes)</b><br><br><br>
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<h1>Acetyl esterase(Aes)</h1>
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<b>Colorimetric response in liquid culture</b>
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<b>Colorimetric response on LB-Agar</b>
[[File:Magenta_butyrate_on_aes_t7_strain.JPG|left|124px|thumb|<b>Fig.1: Magenta butyrate on Aes expressing colony</b>]]
[[File:Magenta_butyrate_on_aes_t7_strain.JPG|left|124px|thumb|<b>Fig.1: Magenta butyrate on Aes expressing colony</b>]]
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<b>Glycoside hydrolase(NagZ)</b><br><br><br>
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<h1>Beta-glucuronidase(GusA)</h1>
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[[File:XGlcNAc_on_nagZ_t7_strain.JPG|left|padding 20px|150px|thumb|<b>Fig.4: X-Glucnac on NagZ expressing colony</b>]]
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<b>Colorimetric response in liquid culture</b>
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<b>Colorimetric response on LB-Agar</b>
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<b>Beta-glucuronidase(GusA)</b><br><br><br>
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[[File:Salmon_glcUA_on_gusA_t7_strain.JPG |left|140px|thumb|<b>Fig.3 Magenta glucuronide on GusA expressing colony</b>]]
[[File:Salmon_glcUA_on_gusA_t7_strain.JPG |left|140px|thumb|<b>Fig.3 Magenta glucuronide on GusA expressing colony</b>]]
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Revision as of 13:03, 13 September 2013

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Contents

What about those hydrolases ?

We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).

On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.

Beta-galactosidase(LacZ)

Colorimetric response in liquid culture Colorimetric response on LB-Agar

Fig.5: X-Gal on LacZ expressing colony
Fig.6: Beta-green-X-gal on LacZ expressing colony


Alkaline phosphatase(PhoA)

Colorimetric response in liquid culture Colorimetric response on LB-Agar

Fig.2: NPP on PhoA expressing colony


Acetyl esterase(Aes)

Colorimetric response in liquid culture Colorimetric response on LB-Agar

Fig.1: Magenta butyrate on Aes expressing colony


Beta-glucuronidase(GusA)

Colorimetric response in liquid culture Colorimetric response on LB-Agar

Fig.3 Magenta glucuronide on GusA expressing colony

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