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Team:ETH Zurich/Experiments 7
From 2013.igem.org
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<b>Colorimetric response in liquid culture</b><br><br> | <b>Colorimetric response in liquid culture</b><br><br> | ||
<b>Colorimetric response on LB-Agar</b> | <b>Colorimetric response on LB-Agar</b> | ||
| - | [[File:XGAL.png|left| | + | [[File:XGAL.png|left|350px|thumb|<b>Fig.5: X-Gal on LacZ expressing colony</b>]] |
| - | [[File:Green_beta_gal_on_t7_strain.JPG|left| | + | [[File:Green_beta_gal_on_t7_strain.JPG|left|350px|thumb|<b>Fig.6: Beta-green-X-gal on LacZ expressing colony</b>]] |
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<b>Colorimetric response in liquid culture</b><br><br> | <b>Colorimetric response in liquid culture</b><br><br> | ||
<b>Colorimetric response on LB-Agar</b> | <b>Colorimetric response on LB-Agar</b> | ||
| - | [[File:PNPP_on_phoA_t7_strain.JPG|left| | + | [[File:PNPP_on_phoA_t7_strain.JPG|left|350px|thumb|<b>Fig.2: NPP on PhoA expressing colony</b>]] |
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<b>Colorimetric response in liquid culture</b><br><br> | <b>Colorimetric response in liquid culture</b><br><br> | ||
<b>Colorimetric response on LB-Agar</b> | <b>Colorimetric response on LB-Agar</b> | ||
| - | [[File:Magenta_butyrate_on_aes_t7_strain.JPG|left| | + | [[File:Magenta_butyrate_on_aes_t7_strain.JPG|left|324px|thumb|<b>Fig.1: Magenta butyrate on Aes expressing colony</b>]] |
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<b>Colorimetric response in liquid culture</b><br><br> | <b>Colorimetric response in liquid culture</b><br><br> | ||
<b>Colorimetric response on LB-Agar</b> | <b>Colorimetric response on LB-Agar</b> | ||
| - | [[File:Salmon_glcUA_on_gusA_t7_strain.JPG |left| | + | [[File:Salmon_glcUA_on_gusA_t7_strain.JPG |left|340px|thumb|<b>Fig.3 Magenta glucuronide on GusA expressing colony</b>]] |
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Revision as of 13:21, 13 September 2013
Contents |
What about those hydrolases ?
We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).
On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.
Beta-galactosidase(LacZ)
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
Alkaline phosphatase(PhoA)
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
Acetyl esterase(Aes)
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
Beta-glucuronidase(GusA)
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
β-N-Acetylglucosaminidase(NagZ)
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
