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Team:ETH Zurich/Experiments 7
From 2013.igem.org
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<h1>Beta-galactosidase(LacZ)</h1> | <h1>Beta-galactosidase(LacZ)</h1> | ||
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[[File:lacZhydro.png|700px|]] | [[File:lacZhydro.png|700px|]] | ||
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<h1>Alkaline phosphatase(PhoA)</h1> | <h1>Alkaline phosphatase(PhoA)</h1> | ||
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| + | <b>Chemical conversion</b> | ||
[[File:phoAhydro.png|700px|]] | [[File:phoAhydro.png|700px|]] | ||
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<h1>Acetyl esterase(Aes)</h1> | <h1>Acetyl esterase(Aes)</h1> | ||
| + | <b>Chemical conversion</b> | ||
[[File:Aeshydro.png|700px|]] | [[File:Aeshydro.png|700px|]] | ||
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<h1>Beta-glucuronidase(GusA)</h1> | <h1>Beta-glucuronidase(GusA)</h1> | ||
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| + | <b>Chemical conversion</b> | ||
[[File:GusAhydro.png|700px|]] | [[File:GusAhydro.png|700px|]] | ||
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<h1>β-N-Acetylglucosaminidase(NagZ)</h1> | <h1>β-N-Acetylglucosaminidase(NagZ)</h1> | ||
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| + | <b>Chemical conversion</b> | ||
[[File:nagZhydro.png|700px|]] | [[File:nagZhydro.png|700px|]] | ||
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Revision as of 13:22, 13 September 2013
Contents |
What about those hydrolases ?
We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).
On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.
Beta-galactosidase(LacZ)
Chemical conversion
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
Alkaline phosphatase(PhoA)
Chemical conversion
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
Acetyl esterase(Aes)
Chemical conversion
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
Beta-glucuronidase(GusA)
Chemical conversion
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
β-N-Acetylglucosaminidase(NagZ)
Chemical conversion
Colorimetric response in liquid culture
Colorimetric response on LB-Agar
