Team:Calgary/Sandbox/Notebook/Protocols/GlycerolStocks

From 2013.igem.org

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<li>Overnight bacterial growth </li>
<li>Overnight bacterial growth </li>
<li>Screw Cap Tubes </li>
<li>Screw Cap Tubes </li>
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</ul>
<li>Glycerol </li>
<li>Glycerol </li>
</ul>
</ul>

Revision as of 06:12, 18 September 2013

Glycerol stocks

Reagents and Materials

  • Overnight bacterial growth
  • Screw Cap Tubes
  • Glycerol

    • Protocol

  • Pipet 0.5mL of glycerol into two 1.5mL screw cap tubes
  • Add 0.5mL of overnight culture to each tube
  • Pipet up and down to gently mix
  • Place one tube in -20°C freezer
  • Place the other tube in -80°C freezer

    • Retrieved from "http://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GlycerolStocks"