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Team:Calgary/Sandbox/Notebook/Protocols/GlycerolStocks
From 2013.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
<li>Overnight bacterial growth </li> | <li>Overnight bacterial growth </li> | ||
<li>Screw Cap Tubes </li> | <li>Screw Cap Tubes </li> | ||
| - | |||
<li>Glycerol </li> | <li>Glycerol </li> | ||
</ul> | </ul> | ||
<h2>Protocol</h2> | <h2>Protocol</h2> | ||
| + | <ol> | ||
<li>Pipet 0.5mL of glycerol into two 1.5mL screw cap tubes</li> | <li>Pipet 0.5mL of glycerol into two 1.5mL screw cap tubes</li> | ||
<li>Add 0.5mL of overnight culture to each tube</li> | <li>Add 0.5mL of overnight culture to each tube</li> | ||
Latest revision as of 06:13, 18 September 2013
Glycerol stocks
Reagents and Materials
- Overnight bacterial growth
- Screw Cap Tubes
- Glycerol
Protocol
- Pipet 0.5mL of glycerol into two 1.5mL screw cap tubes
- Add 0.5mL of overnight culture to each tube
- Pipet up and down to gently mix
- Place one tube in -20°C freezer
- Place the other tube in -80°C freezer
