Team:UANL Mty-Mexico/Wetlab

We divided our circuit in sub-circuits, or modules. Each module comprises a single function of our system. These modules are:

• The RFP switch.- this switch comprises the 37°C thermometer and an RFP reporter right downstream from it.
• The GFP switch.- this switch is similar to the RFP one, but has a 32°C thermometer and a GFP reporter.
• The LacI-GFP switch.- in this switch, a 37°C thermometer is regulating the expression of a LacI gene, which in turn is regulating the expression of a GFP reporter. This GFP reporter is also under the regulation of a 32°C thermometer. In this way, we expect to see two different states: OFF at temperatures below 32°C; ON when temperature is between 32°C and 37°C; and oFF again when temperature is above 37°C.
• The TetR-RFP switch.- here, an RFP reporter is regulated by a 37°C thermometer and a pTet promoter; this switch also includes a TetR construction.
• The cI-TetR-RFP switch.- this switch is similar to the previous one, but also includes a cassette that expresses a thermolabile version of cI. In this way, the expression of RFP will be ON only at temperatures between 37°C and 42°C and OFF at other temperatures.

ISource of intense metagenomic diversity

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Cuatro ciénegas. Source of intense metagenomic diversity

Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.