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Team:Colombia Uniandes/ChimiJournal
From 2013.igem.org
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13th June 2013 | 13th June 2013 | ||
| - | Take out plasmid from the iGEM | + | |
| + | Take out plasmid from the iGEM plaque. | ||
<ol> | <ol> | ||
<li>From the 2013 kit, Plate 1, Well 19 – o</li> | <li>From the 2013 kit, Plate 1, Well 19 – o</li> | ||
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<li>When the water has a dark red color, transfer it to a PCR eppendorf and put on ice.</li> | <li>When the water has a dark red color, transfer it to a PCR eppendorf and put on ice.</li> | ||
</ul> | </ul> | ||
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Electroporation: | Electroporation: | ||
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Use an ice tray with ice to take out the competent cells. The cells are in the Biophysics revco, the one shared with the LAMFU on the second floor of the J building. The revco is opened with the keys of the Biophysics keyring and the cells are on the top right drawer, in the middle. (Look at figure 1) | Use an ice tray with ice to take out the competent cells. The cells are in the Biophysics revco, the one shared with the LAMFU on the second floor of the J building. The revco is opened with the keys of the Biophysics keyring and the cells are on the top right drawer, in the middle. (Look at figure 1) | ||
</html> | </html> | ||
Revision as of 00:17, 25 September 2013
13th June 2013
Take out plasmid from the iGEM plaque.
- From the 2013 kit, Plate 1, Well 19 – o
- From the 2013 kit, Plate 1, Well 2 – i
- From the 2013 kit, Plate 3, Well 17 – c
- 20 µ of miliQ water (ultra pure). Find the plate and stick the tip with water into the well, perforating the aluminum.
- Resuspend the well’s content by gentle pipetting.
- When the water has a dark red color, transfer it to a PCR eppendorf and put on ice.
