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Team:Paris Saclay/Notebook/July/8
From 2013.igem.org
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* Well 1 : 6µL DNA Ladder | * Well 1 : 6µL DNA Ladder | ||
| - | * Well 2 : 5µL Bba_K592009 clone 1 digested by EcoRI+ | + | * Well 2 : 5µL Bba_K592009 clone 1 digested by EcoRI+1µL of 6X loading dye |
| - | * Well 3 : 5µL Bba_K592009 clone 2 digested by EcoRI+ | + | * Well 3 : 5µL Bba_K592009 clone 2 digested by EcoRI+1µL of 6X loading dye |
| - | * Well 4 : 5µL Bba_K592009 clone 1 digested by NotI+ | + | * Well 4 : 5µL Bba_K592009 clone 1 digested by NotI+1µL of 6X loading dye |
| - | * Well 5 : 5µL Bba_K592009 clone 2 digested by NotI+ | + | * Well 5 : 5µL Bba_K592009 clone 2 digested by NotI+1µL of 6X loading dye |
| - | * Well 6 : 5µL Bba_K592009 clone 1 digested by XhoI+ | + | * Well 6 : 5µL Bba_K592009 clone 1 digested by XhoI+1µL of 6X loading dye |
| - | * Well 7 : 5µL Bba_K592009 clone 2 digested by XhoI+ | + | * Well 7 : 5µL Bba_K592009 clone 2 digested by XhoI+1µL of 6X loading dye |
| - | * Well 8 : 5µL Bba_I732017 clone 1 digested by EcoRI/PstI+ | + | * Well 8 : 5µL Bba_I732017 clone 1 digested by EcoRI/PstI+1µL of 6X loading dye |
| - | * Well 9 : 5µL Bba_I732017 clone 2 digested by EcoRI/PstI+ | + | * Well 9 : 5µL Bba_I732017 clone 2 digested by EcoRI/PstI+1µL of 6X loading dye |
* Well 10 : - | * Well 10 : - | ||
* Well 11 : - | * Well 11 : - | ||
Revision as of 17:35, 26 September 2013
Contents |
Notebook : July 8
Lab work
A - Aerobic/Anaerobic regulation system
Objective : Bba_K1155003, Bba_K1155007
1 - Digestion of Bba_K592009, Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
Abou, Anaïs, Sheng, Zhou
Used quantities :
- NotI :
- Bba_K592009 : 2µL
- Buffer orange : 2µL
- NotI : 0.5µL
- H2O : 15.5µL
- XhoI :
- Bba_K592009 : 2µL
- Buffer red : 2µL
- XhoI : 0.5µL
- H2O : 15.5µL
- EcoRI :
- Bba_K592009 : 2µL
- Buffer orange : 2µL
- EcoRI : 0.5µL
- H2O : 15.5µL
- EcoRI/PstI :
- Bba_I732017 : 2µL
- Buffer orange : 2µL
- EcoRI : 0.5µL
- PstI : 0.5µL
- H2O : 15µL
We let our digestion 1h30 at 37°C.
2 - Electrophoresis of the digestion of Bba_K592009, Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
Abdou, Anaïs, Sheng, Zhou
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Expected sizes :
- Bba_K592009 digested by NotI : 2046bp + 693bp
- Bba_K592009 digested by XhoI : 1842bp + 892bp
...
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we obtain fragments at the right size. Our extraction was good. |
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