Team:Marburg/Notebook:August

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<a name="02-08-2013">02.08.2013</a>
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    <legend>Sequencing</legend>
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<span class="inv">Investigator:</span>
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<span class="inv-names">Dominik</span>
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<span class="aim">Aim:</span>
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<span class="aim-desc">Analysis of the sequence of pSB1A3-iBB496315.</span>
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<p>Obviously the wrong plasmid was sent out for sequencing. Due to the lack of enough plasmids for an additional sequence analysis, we will redo a new miniprep.</p>
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    <legend>Transformation</legend>
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    <div class="investigator">
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<span class="inv">Investigator:</span>
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<span class="inv-names">Dominik</span>
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    <div class="aim">
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<span class="aim">Aim:</span>
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<span class="aim-desc">Transformation of <i>E. coli</i> DH5&#945; with the plasmid DNA pSB1A3-iBB4+iBB10+iBB96315 and pSB1A3-iBB4+iBB13+iBB96315.</span>
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<p>The chemo competent <i>E. coli</i> DH5&alpha; cells were transformed with the plasmids pSB1A3-iBB4+iBB10+iBB96315 and pSB1A3-iBB4+iBB13+iBB96315 and plated on LB-Amp-plates.<br />
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The plates were incubated over night at 37° C.</p>
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Revision as of 22:14, 30 September 2013

Notebook: August

02.08.2013

Sequencing
Investigator: Dominik
Aim: Analysis of the sequence of pSB1A3-iBB496315.

Obviously the wrong plasmid was sent out for sequencing. Due to the lack of enough plasmids for an additional sequence analysis, we will redo a new miniprep.

Transformation
Investigator: Dominik
Aim: Transformation of E. coli DH5α with the plasmid DNA pSB1A3-iBB4+iBB10+iBB96315 and pSB1A3-iBB4+iBB13+iBB96315.

The chemo competent E. coli DH5α cells were transformed with the plasmids pSB1A3-iBB4+iBB10+iBB96315 and pSB1A3-iBB4+iBB13+iBB96315 and plated on LB-Amp-plates.
The plates were incubated over night at 37° C.

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