Team:Marburg/Notebook:July
From 2013.igem.org
(Difference between revisions)
Line 86: | Line 86: | ||
</fieldset> | </fieldset> | ||
+ | <div class="notebooky-entry"> | ||
+ | <h2 class="title"> | ||
+ | <a name="23-07-2013">23.07.2013</a> | ||
+ | </h2> | ||
+ | <fieldset class="experiment digest"> | ||
+ | <legend><a name="dig">Digest</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Test digest of the plasmids pSB1C3-iBB4+iBB11+iBB9, pSB1C3-iBB4+iBB12+iBB9 with <i>Eco</i>RI and <i>Pst</i>I</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p><ul class="digest"> | ||
+ | <li>1 µl Plasmid (pSB1C3-iBB4+iBB11+iBB9 and pSB1C3-iBB4+iBB12+iBB9)</li> | ||
+ | <li>0.3 µl EcoRI</li> | ||
+ | <li>0.3 µl PstI</li> | ||
+ | <li>1 µl CutSmart</li> | ||
+ | <li>7.4 µl ddH2O</li> | ||
+ | </ul> | ||
+ | <p>The samples were incubated for 1 h at 37° C.</p> | ||
+ | <table class="gel digest"> | ||
+ | <colgroup> | ||
+ | <col width="50%" /> | ||
+ | <col width="50%" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th colspan="2" class="title">Gel electrophoresis</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" /> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | <span class="gel-elc">Gel substances</span> | ||
+ | <ul class="gel-sub"> | ||
+ | <li>1% Agarose gel</li> | ||
+ | <li>10 µl RedSafe in 50 ml gel</li> | ||
+ | <li>x µl Hyper Ladder</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p> | ||
+ | <span class="exp">Expactations</span> | ||
+ | <ul class="exp"> | ||
+ | <li>Lane 1: 5300 kbp</li> | ||
+ | <li>Lane 2: 3300 kbp</li> | ||
+ | <li>Lane 3: 1300 kbp</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p>Worked as expected.</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | </div> | ||
+ | |||
+ | <div class="notebooky-entry"> | ||
+ | <h2 class="title"> | ||
+ | <a name="24-07-2013">24.07.2013</a> | ||
+ | </h2> | ||
+ | |||
+ | <fieldset class="experiment inoculation"> | ||
+ | <legend><a name="ino">Inoculation</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">inoculation of colonies for plasmid preparation</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p>4 colonies of pSB1C3-iBB4+iBB10+iBB9 and 4 colonies of pSB1C3-iBB4+iBB13+iBB9 were inoculated in 5 ml LB medium containing chloramphenicol.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment digest"> | ||
+ | <legend><a name="dig">Digest</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Digest of pSB1C3-iBB4+iBB11+iBB9, pSB1C3-iBB4+iBB12+iBB9, pSB1C3-iBB6315 and pSB1A3.</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p><ul class="digest"> | ||
+ | <li>1000 ng Plasmid (pSB1C3-iBB4+iBB11+iBB9, pSB1C3-iBB4+iBB12+iBB9)</li> | ||
+ | <li>0.5 µl EcoRI</li> | ||
+ | <li>0.5 µl SpeI</li> | ||
+ | <li>2 µl CutSmart</li> | ||
+ | <li>ad 20 µl ddH2O</li> | ||
+ | </ul> | ||
+ | |||
+ | <p><ul class="digest"> | ||
+ | <li>1000 ng Plasmid (pSB1C3-iBB6315)</li> | ||
+ | <li>0.5 µl XbaI</li> | ||
+ | <li>0.5 µl PstI</li> | ||
+ | <li>2 µl CutSmart</li> | ||
+ | <li>ad 20 µl ddH2O</li> | ||
+ | </ul> | ||
+ | |||
+ | <p><ul class="digest"> | ||
+ | <li>1000 ng Plasmid (pSB1A3)</li> | ||
+ | <li>0.5 µl EcoRI</li> | ||
+ | <li>0.5 µl PstI</li> | ||
+ | <li>2 µl CutSmart</li> | ||
+ | <li>ad 20 µl ddH2O</li> | ||
+ | </ul> | ||
+ | |||
+ | <p>The samples were incubated for 1 h at 37° C.</p> | ||
+ | <table class="gel digest"> | ||
+ | <colgroup> | ||
+ | <col width="50%" /> | ||
+ | <col width="50%" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th colspan="2" class="title">Gel electrophoresis</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" /> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | <span class="gel-elc">Gel substances</span> | ||
+ | <ul class="gel-sub"> | ||
+ | <li>1% Agarose gel</li> | ||
+ | <li>10 µl RedSafe in 50 ml gel</li> | ||
+ | <li>x µl Hyper Ladder</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p> | ||
+ | <span class="exp">Expactations</span> | ||
+ | <ul class="exp"> | ||
+ | <li>Lane 1: 5300 kbp</li> | ||
+ | <li>Lane 2: 3300 kbp</li> | ||
+ | <li>Lane 3: 1300 kbp</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p>All expected fragments were present. The relevant fragments were cut from the gel and then purified via “Qiagen Gel Extraction Kit” (Qiagen, Düsseldorf).</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment ligation"> | ||
+ | <legend><a name="lig">Ligation</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Assemble the biobricks iBB4+iBB11+iBB96315 and iBB4+iBB12+iBB96315 into the vector pSB1A3.</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p><ul class="lig"> | ||
+ | <li>2 µl vector DNA (pSB1A3)</li> | ||
+ | <li>5 µl insert 1 DNA (iBB6315)</li> | ||
+ | <li>5 µl insert 2 DNA (iBB4+iBB11+iBB9, iBB4+iBB12+iBB9)</li> | ||
+ | <li>2 µl 10x T4 DNA ligase buffer</li> | ||
+ | <li>2 µl T4 DNA ligase</li> | ||
+ | <li>4 µl ddH2O</li> | ||
+ | </ul> | ||
+ | <p>The samples were incubated for 2h at 16 °C.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment transformation"> | ||
+ | <legend>Transformation</legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Transformation of <i>E. coli</i> DH5α with the plasmid DNA pSB1A3-iBB4+iBB11+iBB96315 and pSB1A3-iBB4+iBB12+iBB96315.</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p>The chemo competent <i>E. coli</i> DH5α cells were transformed with the plasmids pSB1A3-iBB4+iBB11+iBB96315 and pSB1A3-iBB4+iBB12+iBB96315 DNA and plated on LB-Amp-plates.<br /> | ||
+ | The plates were incubated over night at 37° C.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | </div> | ||
+ | |||
+ | <div class="notebooky-entry"> | ||
+ | <h2 class="title"> | ||
+ | <a name="25-07-2013">25.07.2013</a> | ||
+ | </h2> | ||
+ | |||
+ | <fieldset class="experiment sequencing"> | ||
+ | <legend>Sequencing</legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Complete sequencing of pSB1A3-iBB496315.</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p>8 new sequencing samples were sent out.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment miniprep"> | ||
+ | <legend><a name="min">Miniprep</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">preparation of the plasmids pSB1A3-iBB4+iBB10+iBB9 and pSB1A3-iBB4+iBB13+iBB9.</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p>The plasmids were isolated with the “QIAprep Spin Miniprep Kit” (Qiagen, Düsseldorf) according to the producer instructions.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment digest"> | ||
+ | <legend><a name="dig">Digest</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Test digest of pSB1C3-iBB4+iBB10+iBB9 and pSB1C3-iBB4+iBB13+iBB9 with <i>Eco</i>RI and <i>Pst</i>I</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p><ul class="digest"> | ||
+ | <li>1 µl Plasmid (pSB1C3-iBB4+iBB10+iBB9 and pSB1C3-iBB4+iBB13+iBB9)</li> | ||
+ | <li>0.3 µl EcoRI</li> | ||
+ | <li>0.3 µl PstI</li> | ||
+ | <li>1 µl CutSmart</li> | ||
+ | <li>7.4 µl ddH2O</li> | ||
+ | </ul> | ||
+ | <p>The samples were incubated for 1 h at 37° C.</p> | ||
+ | <table class="gel digest"> | ||
+ | <colgroup> | ||
+ | <col width="50%" /> | ||
+ | <col width="50%" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th colspan="2" class="title">Gel electrophoresis</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" /> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | <span class="gel-elc">Gel substances</span> | ||
+ | <ul class="gel-sub"> | ||
+ | <li>1% Agarose gel</li> | ||
+ | <li>10 µl RedSafe in 50 ml gel</li> | ||
+ | <li>x µl Hyper Ladder</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p> | ||
+ | <span class="exp">Expactations</span> | ||
+ | <ul class="exp"> | ||
+ | <li>Lane 1: 5300 kbp</li> | ||
+ | <li>Lane 2: 3300 kbp</li> | ||
+ | <li>Lane 3: 1300 kbp</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p>Two samples of each plasmid preparation showed the expected fragments.</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment inoculation"> | ||
+ | <legend><a name="ino">Inoculation</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">inoculation of colonies for plasmid preparation</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p>4 colonies of pSB1A3-iBB4+iBB11+iBB96315 and 4 colonies of pSB1A3-iBB4+iBB12+iBB96315 were inoculated in 5 ml LB medium containing chloramphenicol.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment competent cells"> | ||
+ | <legend>Competent cells</legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Overnight culture for making new aliquots of competent cells.</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p>For making new aliquots of <i>E. coli</i> DH5α cells 50 ml LB medium were inoculated with 500 µl of an <i>E. coli</i> DH5α culture.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | </div> | ||
+ | |||
+ | <div class="notebooky-entry"> | ||
+ | <h2 class="title"> | ||
+ | <a name="26-07-2013">26.07.2013</a> | ||
+ | </h2> | ||
+ | |||
+ | <fieldset class="experiment digest"> | ||
+ | <legend><a name="dig">Digest</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Digest of pSB1C3-iBB4+iBB10+iBB9, pSB1C3-iBB4+iBB13+iBB9, pSB1C3-iBB6315 and pSB1A3.</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p><ul class="digest"> | ||
+ | <li>1000 ng Plasmid (pSB1C3-iBB4+iBB10+iBB9, pSB1C3-iBB4+iBB13+iBB9)</li> | ||
+ | <li>0.5 µl EcoRI</li> | ||
+ | <li>0.5 µl SpeI</li> | ||
+ | <li>2 µl CutSmart</li> | ||
+ | <li>ad 20 µl ddH2O</li> | ||
+ | </ul> | ||
+ | |||
+ | <p><ul class="digest"> | ||
+ | <li>1000 ng Plasmid (pSB1C3-iBB6315)</li> | ||
+ | <li>0.5 µl XbaI</li> | ||
+ | <li>0.5 µl PstI</li> | ||
+ | <li>2 µl CutSmart</li> | ||
+ | <li>ad 20 µl ddH2O</li> | ||
+ | </ul> | ||
+ | |||
+ | <p><ul class="digest"> | ||
+ | <li>1000 ng Plasmid (pSB1A3)</li> | ||
+ | <li>0.5 µl EcoRI</li> | ||
+ | <li>0.5 µl PstI</li> | ||
+ | <li>2 µl CutSmart</li> | ||
+ | <li>ad 20 µl ddH2O</li> | ||
+ | </ul> | ||
+ | |||
+ | <p>The samples were incubated for 1 h at 37° C.</p> | ||
+ | <table class="gel digest"> | ||
+ | <colgroup> | ||
+ | <col width="50%" /> | ||
+ | <col width="50%" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th colspan="2" class="title">Gel electrophoresis</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" /> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | <span class="gel-elc">Gel substances</span> | ||
+ | <ul class="gel-sub"> | ||
+ | <li>1% Agarose gel</li> | ||
+ | <li>10 µl RedSafe in 50 ml gel</li> | ||
+ | <li>x µl Hyper Ladder</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p> | ||
+ | <span class="exp">Expactations</span> | ||
+ | <ul class="exp"> | ||
+ | <li>Lane 1: 5300 kbp</li> | ||
+ | <li>Lane 2: 3300 kbp</li> | ||
+ | <li>Lane 3: 1300 kbp</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p>All expected fragments were present. The relevant fragments were cut from the gel and then purified via “Qiagen Gel Extraction Kit” (Qiagen, Düsseldorf).</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment ligation"> | ||
+ | <legend><a name="lig">Ligation</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Assemble the biobricks iBB4+iBB10+iBB9, iBB4+iBB13+iBB9 and iBB96315 into the vector pSB1A3.</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p><ul class="lig"> | ||
+ | <li>2 µl vector DNA (pSB1A3)</li> | ||
+ | <li>5 µl insert 1 DNA (iBB96315)</li> | ||
+ | <li>5 µl insert 2 DNA (iBB4+iBB10+iBB9,iBB4+iBB13+iBB9)</li> | ||
+ | <li>2 µl 10x T4 DNA ligase buffer</li> | ||
+ | <li>2 µl T4 DNA ligase</li> | ||
+ | <li>4 µl ddH2O</li> | ||
+ | </ul> | ||
+ | <p>The samples were incubated for 1h at 16 °C.</p> | ||
+ | <p>Resulting in the plasmids pSB1A3-iBB4+iBB10+iBB96315 and pSB1A3-iBB4+iBB13+iBB96315.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment miniprep"> | ||
+ | <legend><a name="min">Miniprep</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">preparation of the pSB1A3-iBB4+iBB11+iBB96315 and pSB1A3-iBB4+iBB12+iBB96315.</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p>The plasmids were isolated with the “QIAprep Spin Miniprep Kit” (Qiagen, Düsseldorf) according to the producer instructions.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment digest"> | ||
+ | <legend><a name="dig">Digest</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Test digest of ppSB1A3-iBB4+iBB11+iBB96315 and pSB1A3-iBB4+iBB12+iBB96315 with <i>Eco</i>RI and <i>Pst</i>I</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p><ul class="digest"> | ||
+ | <li>1 µl Plasmid (pSB1C3-iBB39,pSB1A3-iBB49 and pSB1C3-iBB6315)</li> | ||
+ | <li>0.3 µl EcoRI</li> | ||
+ | <li>0.3 µl PstI</li> | ||
+ | <li>1 µl CutSmart</li> | ||
+ | <li>7.4 µl ddH2O</li> | ||
+ | </ul> | ||
+ | <p>The samples were incubated for 1 h at 37° C.</p> | ||
+ | <table class="gel digest"> | ||
+ | <colgroup> | ||
+ | <col width="50%" /> | ||
+ | <col width="50%" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th colspan="2" class="title">Gel electrophoresis</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" /> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | <span class="gel-elc">Gel substances</span> | ||
+ | <ul class="gel-sub"> | ||
+ | <li>1% Agarose gel</li> | ||
+ | <li>10 µl RedSafe in 50 ml gel</li> | ||
+ | <li>x µl Hyper Ladder</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p> | ||
+ | <span class="exp">Expactations</span> | ||
+ | <ul class="exp"> | ||
+ | <li>Lane 1: 5300 kbp</li> | ||
+ | <li>Lane 2: 3300 kbp</li> | ||
+ | <li>Lane 3: 1300 kbp</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p>Most of the preparations resulted in the expected fragments, all others were discarded.</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | |||
+ | <fieldset class="experiment competent cells"> | ||
+ | <legend>Competent cells</legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">New aliquots of competent cells (<i>E. coli</i> DH5α).</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p>About 80 new aliquots were made.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | </div> | ||
</html> | </html> |
Revision as of 22:22, 30 September 2013