Team:ETH Zurich/Experiments 6

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[[File:Grid.png|200px|right|thumb||<b>Figure 1. Gameplay with GFP receiver construct </b>]]<p align="justify" >The receiver and sender colonies were plated in the honeycomb hexagonal pattern on LB-Agar for a timelapse experiment to follow the OHHL diffusion from sender cells to receiver cells.<br> The mine cells/sender cells are represented by green circles, the numbers indicate how many mines surround the non mine colonies . The grey-scale makes it easy to identify 1,2 or 3 surrounding mines. As predicted in our model a gradient is visible in the colony. This gives the player information about the location of the mine. Based on this information , the player can decide about the next move, as in to which colony the substrate needs to be added in order to move past the mines without detonating them , or to flag them.</p>
[[File:Grid.png|200px|right|thumb||<b>Figure 1. Gameplay with GFP receiver construct </b>]]<p align="justify" >The receiver and sender colonies were plated in the honeycomb hexagonal pattern on LB-Agar for a timelapse experiment to follow the OHHL diffusion from sender cells to receiver cells.<br> The mine cells/sender cells are represented by green circles, the numbers indicate how many mines surround the non mine colonies . The grey-scale makes it easy to identify 1,2 or 3 surrounding mines. As predicted in our model a gradient is visible in the colony. This gives the player information about the location of the mine. Based on this information , the player can decide about the next move, as in to which colony the substrate needs to be added in order to move past the mines without detonating them , or to flag them.</p>
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<h1>The game with the hydrolases receiver construct</h1>
 
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[[File:Circuit_Minesweeper.png|630px|left|thumb|<b>Figure 3. [OHHL] dependent information processing</b>]]
 
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<p align="justify"> 1. Receiver cells signal no mines in their vicinity. If there is no mine adjacent to a non-mine colony, no OHHL is reached to the receiver cell and so no OOHL is processed. In this case, a constitutive hydrolase is expressed which on addition of the multi-substrate will give a yellow color. This indicates that there are no mines adjacent to this colony.
 
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2.Receiver cells respond to low OHHL levels: If one mine cell is close to a non-mine colony and by OHHL diffusion takes place to reach the non-mine colony. Due to a bandpass filter using the LuxR system in the non-mine colony,low concentrations of OHHL is detected which activates a reporter. Addition of the multi-substrate to this colony produces a salmon color and this indicates one mine is adjacent to the colony just played.
 
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3.Receiver cells respond to high OHHL levels: If there are two or more mine colonies adjacent to a non-mine colony, a higher concentration of diffused OHHL will be processed by the receiver colony. The LuxR system works as a bandpass filter again and induces the expression of another reporter to show that 2 or more mines surround the selected colony. Addition of the multi-substrate to this colony produces a magenta color that indicates more than two mines adjacent to this colony.<br>
 
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Revision as of 16:05, 1 October 2013

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80px-Eth igem logo.png

The game with the GFP receiver construct

Figure 1. Gameplay with GFP receiver construct

The receiver and sender colonies were plated in the honeycomb hexagonal pattern on LB-Agar for a timelapse experiment to follow the OHHL diffusion from sender cells to receiver cells.
The mine cells/sender cells are represented by green circles, the numbers indicate how many mines surround the non mine colonies . The grey-scale makes it easy to identify 1,2 or 3 surrounding mines. As predicted in our model a gradient is visible in the colony. This gives the player information about the location of the mine. Based on this information , the player can decide about the next move, as in to which colony the substrate needs to be added in order to move past the mines without detonating them , or to flag them.


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