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Team:Paris Saclay/Notebook/July/10
From 2013.igem.org
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''==== |
| - | ===='''1 - Transformation of | + | ===='''1 - Transformation of BBa_I732019 in DH5α'''==== |
Sheng | Sheng | ||
| - | + | Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]] | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | Protocol : [[Team:Paris_Saclay/Protocols/ | + | |
| - | ===='''Objective : obtaining biobricks in | + | ===='''Objective : obtaining biobricks in pSB3K3'''==== |
| - | ===='''1 - Mini and maxi preparation of the culture of | + | ===='''1 - Mini and maxi preparation of the culture of BBa_J04450'''==== |
Abdou | Abdou | ||
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{| | {| | ||
| style="border:1px solid black;padding:5px;background-color:#DE;" | | | style="border:1px solid black;padding:5px;background-color:#DE;" | | ||
| - | Transformation of | + | Transformation of BBa_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations. |
|} | |} | ||
| - | Protocol : [[Team:Paris_Saclay/ | + | Protocol : [[Team:Paris_Saclay/mini_maxi|Mini and maxi preparation]] |
We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic. | We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic. | ||
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==='''B - PCB sensor system'''=== | ==='''B - PCB sensor system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein'''==== |
| - | ===='''1 - Transformation of ligation of | + | ===='''1 - Transformation of ligation of pSB1C3 and BphA1 or BphR1 or BphR2'''==== |
Abdou | Abdou | ||
| - | Protocol : [[Team:Paris_Saclay/Protocols/ | + | Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]] |
Latest revision as of 16:36, 4 October 2013
Contents |
Notebook : July 10
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155003, BBa_K1155007
1 - Transformation of BBa_I732019 in DH5α
Sheng
Protocol : Bacterial transformation
Objective : obtaining biobricks in pSB3K3
1 - Mini and maxi preparation of the culture of BBa_J04450
Abdou
|
Transformation of BBa_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations. |
Protocol : Mini and maxi preparation
We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
B - PCB sensor system
Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein
1 - Transformation of ligation of pSB1C3 and BphA1 or BphR1 or BphR2
Abdou
Protocol : Bacterial transformation
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