Team:ETH Zurich
From 2013.igem.org
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The goal is to clear an agar “minefield” without detonating the mines. Genetically engineered <i>Escherichia coli</i> colonies are used as mines and non-mines. Mines secrete the signaling molecule AHL whereas non-mines process the signal. To distinguish between AHL-levels, a library of P<sub>LuxR</sub> promoters with various AHL sensitivities was created through site-saturation mutagenesis. High-pass filters were constructed to control the expression of different orthogonal hydrolases in non-mines, depending on the number of surrounding mines.<br><br> The slideshow covers all our topics so feel free to <b>CLICK ON ANY PICTURE</b> in <b>ANY SLIDESHOW</b> to navigate to the according subjects. | The goal is to clear an agar “minefield” without detonating the mines. Genetically engineered <i>Escherichia coli</i> colonies are used as mines and non-mines. Mines secrete the signaling molecule AHL whereas non-mines process the signal. To distinguish between AHL-levels, a library of P<sub>LuxR</sub> promoters with various AHL sensitivities was created through site-saturation mutagenesis. High-pass filters were constructed to control the expression of different orthogonal hydrolases in non-mines, depending on the number of surrounding mines.<br><br> The slideshow covers all our topics so feel free to <b>CLICK ON ANY PICTURE</b> in <b>ANY SLIDESHOW</b> to navigate to the according subjects. | ||
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<li><b><br> The computer game Minesweeper</b><br><br> | <li><b><br> The computer game Minesweeper</b><br><br> | ||
The computer game consists of a square lattice grid with hidden squares that can be mines or non-mines. The goal of the game is to clear the field without detonating the mines. The identity of a square is revealed by left clicking on it: the digit revealed represent the number of adjacent mines. The player uses this information to decide which square can be a potential mine and flags it. A square can be flagged by a right click. The game is won when all the mines and only the mines are flagged. If a mine is encountered, it is detonated along with all other mines and the game is over. | The computer game consists of a square lattice grid with hidden squares that can be mines or non-mines. The goal of the game is to clear the field without detonating the mines. The identity of a square is revealed by left clicking on it: the digit revealed represent the number of adjacent mines. The player uses this information to decide which square can be a potential mine and flags it. A square can be flagged by a right click. The game is won when all the mines and only the mines are flagged. If a mine is encountered, it is detonated along with all other mines and the game is over. | ||
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<li><div></html>{{:Team:ETH Zurich/Templates/Video|width=660px|id=video1|ratio=4/3|srcMP4=<html>https://static.igem.org/mediawiki/2013/7/76/Colisweeperfinal.mp4</html>|poster=<html>/wiki/images/5/5f/Introslide.png</html>}}<html> | <li><div></html>{{:Team:ETH Zurich/Templates/Video|width=660px|id=video1|ratio=4/3|srcMP4=<html>https://static.igem.org/mediawiki/2013/7/76/Colisweeperfinal.mp4</html>|poster=<html>/wiki/images/5/5f/Introslide.png</html>}}<html> | ||
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Revision as of 14:35, 25 October 2013