Team:ETH Zurich/Play
From 2013.igem.org
Line 17: | Line 17: | ||
<h1>Colisweeper gameplay </h1> | <h1>Colisweeper gameplay </h1> | ||
- | [[File:Playthegame2.png| | + | [[File:Playthegame2.png|700px|left|thumb|<b>Figure 2: The Gameplay</b> Choose a colony, play it or flag it and follow the color code logic to win the game!]] |
<p align="justify">To play Colisweeper, the gamer has to <b>plate mines and non mines</b>. After <b> 12 h of incubation</b> the game is ready to be played. The player has to <b> pipette a colorless substrate-mix</b> on a colony on the agar minefield. A single move of pipetting would require the player to choose between two substrates. To "play" a colony, the player pipettes a multi-susbtrate mix, which reveals the identity of the colony- as in the number of mines surrounding a non-mine. If the colony turns yellow, red or violet, this means that zero, one or two mines are adjacent to the "played" colony, respectively. This means you are safe and can perform your next move. However if the colony turns blue, you hit a mine and the game is over. If you want to <b>flag</b> a colony, the single substrate is pipetted onto a colony if the player is certain it is a mine. Upon this the colony turns green. Addition of either substrate produces a defined colored product within minutes, allowing identification of the played colony and the number of mines surrounding it.</p> | <p align="justify">To play Colisweeper, the gamer has to <b>plate mines and non mines</b>. After <b> 12 h of incubation</b> the game is ready to be played. The player has to <b> pipette a colorless substrate-mix</b> on a colony on the agar minefield. A single move of pipetting would require the player to choose between two substrates. To "play" a colony, the player pipettes a multi-susbtrate mix, which reveals the identity of the colony- as in the number of mines surrounding a non-mine. If the colony turns yellow, red or violet, this means that zero, one or two mines are adjacent to the "played" colony, respectively. This means you are safe and can perform your next move. However if the colony turns blue, you hit a mine and the game is over. If you want to <b>flag</b> a colony, the single substrate is pipetted onto a colony if the player is certain it is a mine. Upon this the colony turns green. Addition of either substrate produces a defined colored product within minutes, allowing identification of the played colony and the number of mines surrounding it.</p> | ||
<br clear="all" /> | <br clear="all" /> | ||
{{:Team:ETH_Zurich/templates/footer}} | {{:Team:ETH_Zurich/templates/footer}} |
Revision as of 10:15, 26 October 2013
Contents |
Design of the mine grid for the bio-game
![](/wiki/images/b/b7/Project_description10.png)
Inspired by the computer game Minesweeper, we aspire to incorporate all the same features and rules in the biological version of the game. To replicate the "mouse click" in the computer game, the player adds a substrate to the colony of his or her choice which leads to a colorful enzyme-substrate reaction. The agar mine field consists of colonies which can be one of the following:
a non-mine colony, which will display the "safe colour" when played
a non-mine colony located close to one or two mines, which on addition of a multi-substrate reveals the number of mines in the vicinity of this colony
a mine colony, which when played reveals the mine color and the game is over
You also have the option to flag a mine - if you are certain that a colony is a mine, you can flag it using a second substrate.
Bio-game geometry
The diffusion of the signaling molecules is of course radial, therefor if we plate the bacterial colonies on an agar petridish similar to squares in the computer game the distances between the colonies would not be identical, because the diagonal would be √2cm if the distance between the other colonies in the square is 1cm .Additionally in this case, each colony has a possibility to have eight mine colonies adjacent to it. To mimic the same pattern in a biological setting will be quite complicated, and we would need a lot of different and very precise high pass filters and reporters. To have a simple grid structure, we decided on a grid with a hexagonal honeycomb-like pattern, where colonies are placed at all edges of the hexagon, except the center. In the hexagonal grid, the number of mine colonies around a chosen colony is restricted to a maximal number of three surrounding mines.
Colisweeper gameplay
To play Colisweeper, the gamer has to plate mines and non mines. After 12 h of incubation the game is ready to be played. The player has to pipette a colorless substrate-mix on a colony on the agar minefield. A single move of pipetting would require the player to choose between two substrates. To "play" a colony, the player pipettes a multi-susbtrate mix, which reveals the identity of the colony- as in the number of mines surrounding a non-mine. If the colony turns yellow, red or violet, this means that zero, one or two mines are adjacent to the "played" colony, respectively. This means you are safe and can perform your next move. However if the colony turns blue, you hit a mine and the game is over. If you want to flag a colony, the single substrate is pipetted onto a colony if the player is certain it is a mine. Upon this the colony turns green. Addition of either substrate produces a defined colored product within minutes, allowing identification of the played colony and the number of mines surrounding it.