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Team:Colombia Uniandes/Journal
From 2013.igem.org
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After we went over the project, we proceed to design our primers so we could place our order, taking into account that we are going to use Fusion PCR as our main protocol. Our primers take around 2 weeks to arrive… sooo meanwhilee all the team started making our E.coli babies electrocompetent, so we can work with them later on. | After we went over the project, we proceed to design our primers so we could place our order, taking into account that we are going to use Fusion PCR as our main protocol. Our primers take around 2 weeks to arrive… sooo meanwhilee all the team started making our E.coli babies electrocompetent, so we can work with them later on. | ||
| - | So today we | + | So today we took our bacterial cells (Top 10) onto an LB medium (no antibiotics). We let it ON at 37°C on the shaker. |
===='''26-Jun-2013(AllJournals!)'''==== | ===='''26-Jun-2013(AllJournals!)'''==== | ||
Today we are going to start preparing our cells | Today we are going to start preparing our cells | ||
Revision as of 00:45, 17 July 2013
Dear Journal! :)
Here we will place all the information about the work we have been doing during this time, it will be named by date and project, for example: 14-Mar-2013(NickelJournal) or 14-Mar-2013(GlucoJournal). We hope you enjoy our work and thoughts as much as we do!
24-Jun-2013(AllJournals!)
After we went over the project, we proceed to design our primers so we could place our order, taking into account that we are going to use Fusion PCR as our main protocol. Our primers take around 2 weeks to arrive… sooo meanwhilee all the team started making our E.coli babies electrocompetent, so we can work with them later on.
So today we took our bacterial cells (Top 10) onto an LB medium (no antibiotics). We let it ON at 37°C on the shaker.
26-Jun-2013(AllJournals!)
Today we are going to start preparing our cells
