"
Team:Marburg/Notebook:August
From 2013.igem.org
(Difference between revisions)
Enantiomere (Talk | contribs) |
|||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{:Team:Marburg/Template:Header}} | {{:Team:Marburg/Template:Header}} | ||
{{:Team:Marburg/Template:ContentTitleNav}} | {{:Team:Marburg/Template:ContentTitleNav}} | ||
| - | Notebook: August | + | Notebook: August <html><a href="https://2013.igem.org/Team:Marburg/Notebook:September"><img src="https://static.igem.org/mediawiki/2013/7/71/Mr-igem-next-arrow.png" style="float:right;margin-left:5px !important;" alt="Next"></a> <a href="https://2013.igem.org/Team:Marburg/Notebook:July"><img src="https://static.igem.org/mediawiki/2013/1/13/Mr-igem-previous-arrow.png" alt="Previous" style="float:right;"></a></html> |
| - | {{:Team:Marburg/Template:ContentStartNav}} | + | {{:Team:Marburg/Template:ContentStartNav}}<html> |
| - | + | ||
| - | <html> | + | |
<div class="notebooky-entry"> | <div class="notebooky-entry"> | ||
| Line 42: | Line 40: | ||
<li>0.3 µl <i>Pst</I></li> | <li>0.3 µl <i>Pst</I></li> | ||
<li>1 µl CutSmart</li> | <li>1 µl CutSmart</li> | ||
| - | <li>7.4 µl | + | <li>7.4 µl ddbidest. H2O</li> |
</ul> | </ul> | ||
| - | <p>The samples were incubated for 1 h at | + | <p>The samples were incubated for 1 h at 37 °C.</p> |
<p>After that, a gel electrophoresis was made, but failed and has to be repeated the next day</p> | <p>After that, a gel electrophoresis was made, but failed and has to be repeated the next day</p> | ||
</div> | </div> | ||
| Line 97: | Line 95: | ||
<div class="exp-content"> | <div class="exp-content"> | ||
<p>The chemo competent <i>E. coli</i> DH5α cells were transformed with the plasmids pSB1A3-iBB4+iBB10+iBB96315 and pSB1A3-iBB4+iBB13+iBB96315 and plated on LB-Amp-plates.<br /> | <p>The chemo competent <i>E. coli</i> DH5α cells were transformed with the plasmids pSB1A3-iBB4+iBB10+iBB96315 and pSB1A3-iBB4+iBB13+iBB96315 and plated on LB-Amp-plates.<br /> | ||
| - | The plates were incubated over night at | + | The plates were incubated over night at 37 °C.</p> |
</div> | </div> | ||
</fieldset> | </fieldset> | ||
| Line 135: | Line 133: | ||
<p><ul class="digest"> | <p><ul class="digest"> | ||
<li>1 µl Plasmid (pSB1A3-iBB496315 and iBB4+iBB13+iBB96315)</li> | <li>1 µl Plasmid (pSB1A3-iBB496315 and iBB4+iBB13+iBB96315)</li> | ||
| - | <li>0.3 µl | + | <li>0.3 µl <i>EcoR</i>I</li> |
| - | <li>0.3 µl PstI</li> | + | <li>0.3 µl <i>PstI</i></li> |
<li>1 µl CutSmart</li> | <li>1 µl CutSmart</li> | ||
| - | <li>7.4 µl | + | <li>7.4 µl ddbidest. H2O</li> |
</ul> | </ul> | ||
| - | <p>The samples were incubated for 1 h at | + | <p>The samples were incubated for 1 h at 37 °C.</p> |
<table class="gel digest"> | <table class="gel digest"> | ||
<colgroup> | <colgroup> | ||
| Line 154: | Line 152: | ||
<tr> | <tr> | ||
<td> | <td> | ||
| - | <img src=" | + | <img src="https://static.igem.org/mediawiki/2013/2/26/Gel_2013-08-02.png" width="60%" alt="gel-electrophoresis-image" /> |
</td> | </td> | ||
<td> | <td> | ||
| Line 162: | Line 160: | ||
<li>1% Agarose gel</li> | <li>1% Agarose gel</li> | ||
<li>10 µl RedSafe in 50 ml gel</li> | <li>10 µl RedSafe in 50 ml gel</li> | ||
| - | <li> | + | <li>6 µl GeneRuler™ 1kb plus DNA Ladder (Thermo Scientific)</li> |
</ul> | </ul> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<p>Worked as expected.</p> | <p>Worked as expected.</p> | ||
</td> | </td> | ||
| Line 203: | Line 192: | ||
<li>pSB1A3-iBB4+iBB13+iBB96315: mutation in terminator, will be ignored.</li> | <li>pSB1A3-iBB4+iBB13+iBB96315: mutation in terminator, will be ignored.</li> | ||
</ul> | </ul> | ||
| - | <p>All plasmids are brought to Marian who will transform them | + | <p>All plasmids are brought to Marian who will transform them into <i>P. tricornutum</i> cells.</p> |
</div> | </div> | ||
</fieldset> | </fieldset> | ||
Latest revision as of 11:15, 27 October 2013
Notebook: August 
