Team:Marburg/Notebook:Primer list

From 2013.igem.org

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Supplementary
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Supplementary <html><a href="https://2013.igem.org/Team:Marburg/Notebook:October"><img src="https://static.igem.org/mediawiki/2013/1/13/Mr-igem-previous-arrow.png" alt="Previous" style="float:right;"></a></html>
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{{:Team:Marburg/Template:ContentStartNav}}<html><div class="suppentry">
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<fieldset class="primerlist">
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<h2 class="title">
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   <td height=21 class=xl72 width=44 style='height:15.75pt;width:33pt'>No</td>
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   <th height=21 class=xl72 width=44 style='height:15.75pt;width:33pt'>No</td>
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   <td class=xl73 width=207 style='border-left:none;width:155pt'>Name</td>
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   <th class=xl73 width=207 style='border-left:none;width:155pt'>Name</td>
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   <td class=xl73 width=503 style='border-left:none;width:377pt'>Sequence</td>
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   <th class=xl73 width=503 style='border-left:none;width:377pt'>Sequence</td>
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  </tr>
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  </thead>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR01</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR01</td>
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<td>iBB1</td>
<td>iBB1</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071001 </td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071001" target="_blank">BBa_K1071001</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
<td>ble resistance gene</td>
<td>ble resistance gene</td>
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<td>iBB2</td>
<td>iBB2</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071002</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071002" target="_blank">BBa_K1071002</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
<td>cat resistance gene</td>
<td>cat resistance gene</td>
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<td>iBB3</td>
<td>iBB3</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071003</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071003" target="_blank">BBa_K1071003</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
<td>P<sub>fcpB</sub></td>
<td>P<sub>fcpB</sub></td>
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<td>iBB4</td>
<td>iBB4</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071004</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071004" target="_blank">BBa_K1071004</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>PNR</td>
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<td>P<sub>NR</sub></td>
</tr>
</tr>
<tr>
<tr>
<td>iBB5</td>
<td>iBB5</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
-
<td>BBa_K1071005 </td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071005" target="_blank">BBa_K1071005</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>TfcpA</td>
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<td>T<sub>fcpA</sub></td>
</tr>
</tr>
<tr>
<tr>
<td>iBB6</td>
<td>iBB6</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071006</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071006" target="_blank">BBa_K1071006</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>TNR</td>
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<td>T<sub>NR</sub></td>
</tr>
</tr>
<tr>
<tr>
<td>iBB7</td>
<td>iBB7</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
-
<td>BBa_K1071007</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071007" target="_blank">BBa_K1071007</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
<td>antibody light chain</td>
<td>antibody light chain</td>
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<td>iBB8</td>
<td>iBB8</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071008</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071008" target="_blank">BBa_K1071008</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
<td>antibody heavy chain</td>
<td>antibody heavy chain</td>
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<td>iBB9</td>
<td>iBB9</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071009</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071009" target="_blank">BBa_K1071009</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
<td>eGFP</td>
<td>eGFP</td>
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<td>iBB10</td>
<td>iBB10</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071010</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071010" target="_blank">BBa_K1071010</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>SPTP1</td>
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<td>SPTP1 (Stroma localization)</td>
</tr>
</tr>
<tr>
<tr>
<td>iBB11</td>
<td>iBB11</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071011</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071011" target="_blank">BBa_K1071011</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>SPTP2</td>
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<td>SPTP2 (Stroma localization)</td>
</tr>
</tr>
<tr>
<tr>
<td>iBB12</td>
<td>iBB12</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071012</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071012" target="_blank">BBa_K1071012</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>SP1</td>
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<td>SP1 (ER localization)</td>
</tr>
</tr>
<tr>
<tr>
<td>iBB13</td>
<td>iBB13</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071013</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071013" target="_blank">BBa_K1071013</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>SP2</td>
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<td>SP2 (ER localization)</td>
</tr>
</tr>
<tr>
<tr>
<td>iBB14-15</td>
<td>iBB14-15</td>
<th>&nbsp;</th>
<th>&nbsp;</th>
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<td>BBa_K1071014</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071014" target="_blank">BBa_K1071014</a></td>
<th>&nbsp;</th>
<th>&nbsp;</th>
<td>RFP-MTS</td>
<td>RFP-MTS</td>
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<br />
<br />
<fieldset class="cloningstrategies">
<fieldset class="cloningstrategies">
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<p>Antibody construct (vector)</p>
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<p>Antibody construct (pSB1A3 iBB486476315)</p>
<br />
<br />
<img src="https://static.igem.org/mediawiki/2013/3/34/MR_cloning_antibody.png" width="100%" alt="cloningstrategies" />
<img src="https://static.igem.org/mediawiki/2013/3/34/MR_cloning_antibody.png" width="100%" alt="cloningstrategies" />
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<br />
<br />
<fieldset class="cloningstrategies">
<fieldset class="cloningstrategies">
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<p>Promotor P<sub>NR</sub> strength measurement (vector)</p>
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<p>Promotor P<sub>NR</sub> strength measurement (pSB1A3 iBB496315)</p>
<br />
<br />
<img src="https://static.igem.org/mediawiki/2013/8/87/MR_cloning_PNR.png" width="100%" alt="cloningstrategies" />
<img src="https://static.igem.org/mediawiki/2013/8/87/MR_cloning_PNR.png" width="100%" alt="cloningstrategies" />
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<br />
<br />
<fieldset class="cloningstrategies">
<fieldset class="cloningstrategies">
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<p>Promotor P<sub>fcpB</sub> strength measurement (vector)</p>
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<p>Promotor P<sub>fcpB</sub> strength measurement (pSB1A3 iBB395416)</p>
<br />
<br />
<img src="https://static.igem.org/mediawiki/2013/7/7c/MR_cloning_PfcpB.png" width="100%" alt="cloningstrategies" />
<img src="https://static.igem.org/mediawiki/2013/7/7c/MR_cloning_PfcpB.png" width="100%" alt="cloningstrategies" />
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<br />
<br />
<fieldset class="cloningstrategies">
<fieldset class="cloningstrategies">
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<p>Signal peptide construct (vector)</p>
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<p>Signal peptide constructs (pSB1A3 iBB4-10/11/12/13-96315)</p>
<br />
<br />
<img src="https://static.igem.org/mediawiki/2013/0/08/MR_cloning_signalpeptide.png" width="100%" alt="cloningstrategies" />
<img src="https://static.igem.org/mediawiki/2013/0/08/MR_cloning_signalpeptide.png" width="100%" alt="cloningstrategies" />

Latest revision as of 11:17, 27 October 2013

Supplementary Previous

Primer list


No Name Sequence
iMR01 ble-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCCAAGTTGACCAGTGCCGTTC
iMR02 ble-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCAGTCCTGCTCCTCGGCCACGAAG
iMR03 cat-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGAAAAAAATCACTGGATATA
iMR04 cat-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACGCCCCGCCCTGCCACTCATCGC
iMR05 P_fcbP-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATCCGGAGAGATTCAAAATTCAATC
iMR06 P_fcbP-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTTGACATCTGGCAACCGTGAAATA
iMR07 P_NR-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACATATGCGGAAGTGACTGTAAACGA
iMR08 P_NR-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCGTTCGCACAAGTGGTGACTTTGAA
iMR09 T_fcpA-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACCTTCCTTAAAAATTTAATTTTCAT
iMR10 T_fcpA-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTCGAGAAAACTCATCCTGTGCCTT
iMR11 T_NR-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACAATTTACTTTAAGACTAGC
iMR12 T_NR-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCGGATGCGTTCATTTTAG
iMR13 Cat-bb-Pvu-For CCAGACCGTTCAGCTAGATATTACG
iMR14 Cat-bb-Pvu-Rev CGTAATATCTAGCTGAACGGTCTGG
iMR15 Cat-bb-Eco-For GCTCATCCGGAGTTCCGTATGGC
iMR16 Cat-bb-Eco-Rev GCCATACGGAACTCCGGATGAGC
iMR17 PfcpB-Pvu-For CGACGCTCGCTCCAGCTAGGGCTTCCTTAC
iMR18 PfcpB-Pvu-Rev GTAAGGAAGCCCTAGCTGGAGCGAGCGTCG
iMR19 PNR-Pst-For TTCCGGCACCTACAGCGAGGAGTTTTG
iMR20 PNR-Pst-Rev CAAAACTCCTCGCTGTAGGTGCCGGAA
iMR21 T_fcpa-Xho-For GGATGAGTTTTCTAGAGGCCGGTCTCC
iMR22 T-fcpa-Xho-Rev GGAGACCGGCCTCTAGAAAACTCATCC
iMR23 Seq_P_NR_For CGGGGCTGGCTTAACTAT
iMR24 Seq_P_NR_Rev GTAAATTGAAGCTTGCATGC
iMR25 Seq_T_NR and P_fcpB_For ACTTGTGCGAACGGAATT
iMR26 Seq_T_NR and P_fcpB_Rev CACCGGCGAAGTCGTCCT
iMR27 Seq_Ble_For CTACGACGCTCGCTCCAG
iMR28 Seq_Ble_Rev GGCATCCATGGTGAAGACGA
iMR29 Seq_T_fcpA_For AGATCGGCGAGCAGCCGT
iMR30 Seq_T_fcpA_Rev CCCGCGCGTTGGCCGATT
iMR31 Cl4mA_l_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAAACCCCAGCGCAGCTT
iMR32 Cl4mA_l_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTAACACTCTCCACGGTTGAAGCTC
iMR33 Cl4mA_h_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGTTTGGGCTCAGCTGG
iMR34 Cl4mA_h_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCATTTACCCGGAGACAGGGAG
iMR35 Seq_pJET12_for CGGCGTAATACGACTCACTATA
iMR36 Seq_pJET12_rev ACCTACAACGGTTCCTGATG
iMR37 Seq_for (VF2) TGCCACCTGACGTCTAAGAA
iMR38 Seq_rev (VR) ATTACCGCCTTTGAGTGAGC
iMR39 Seq_cat.int_rev GCTCATGGAAAACGGTGTAA
iMR40 eGFP_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAGG
iMR41 eGFP_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACTTGTACAGCTCGTCC
iMR42 SPTP1_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAGATCCTTTTGCATCG
iMR43 SPTP1_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCATCCATGACAATCGTTG
iMR44 SPTP2_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAAGACTGCTGTCATTGC
iMR45 SPTP2_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTGGCAACCGAAGTACGGG
iMR46 SP1_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGACTGCAATAGGGTCTTT
iMR47 SP1_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCAAGACACACTGCTGTG
iMR48 SP2_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGCGCTCGTTTCTACTTTG
iMR49 SP2_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCAGTCGCGAACGAAGCG
iMR50 RFP_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCTTCCTCCGAAGACG
iMR51 RFP_rev ATATGGATCCAGCACCGGTGGAGTGACG
iMR52 MTS_for ATATGGATCCGCGCCGCAGCGGGAGGAAG
iMR53 MTS_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTACCTTTCTAGGAAAAGTTGGCGC
iMR54 RBS_for AAAGAGGAGAAA


Internal BioBricks - Classification


Internal number   Part name   Description
iBB1   BBa_K1071001   ble resistance gene
iBB2   BBa_K1071002   cat resistance gene
iBB3   BBa_K1071003   PfcpB
iBB4   BBa_K1071004   PNR
iBB5   BBa_K1071005   TfcpA
iBB6   BBa_K1071006   TNR
iBB7   BBa_K1071007   antibody light chain
iBB8   BBa_K1071008   antibody heavy chain
iBB9   BBa_K1071009   eGFP
iBB10   BBa_K1071010   SPTP1 (Stroma localization)
iBB11   BBa_K1071011   SPTP2 (Stroma localization)
iBB12   BBa_K1071012   SP1 (ER localization)
iBB13   BBa_K1071013   SP2 (ER localization)
iBB14-15   BBa_K1071014   RFP-MTS


Cloning strategies


Antibody construct (pSB1A3 iBB486476315)


cloningstrategies


Promotor PNR strength measurement (pSB1A3 iBB496315)


cloningstrategies


Promotor PfcpB strength measurement (pSB1A3 iBB395416)


cloningstrategies


Signal peptide constructs (pSB1A3 iBB4-10/11/12/13-96315)


cloningstrategies


Retrieved from "http://2013.igem.org/Team:Marburg/Notebook:Primer_list"