Team:Uppsala/protocols

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<div id="igem"><a href="https://2013.igem.org/Main_Page"><img class="igem" src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_Uppsala_IGEM_log_blue.png"></a></div>
<div id="igem"><a href="https://2013.igem.org/Main_Page"><img class="igem" src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_Uppsala_IGEM_log_blue.png"></a></div>
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<img class="slogan" src="https://static.igem.org/mediawiki/2013/a/ad/Uppsala2013_Header.png">
</div>  
</div>  
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                                                 <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                     <ul>
                                                     <ul>
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                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">P-coumaric acid</a></li>
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                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">p-Coumaric acid</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
<ul>
<ul>
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<li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">P-Coumaric acid</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">Kinetic model</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/toxicity-model">Toxicity model</a></li>
</ul></li>
</ul></li>
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li>
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                                                <li><a href="https://2013.igem.org/Team:Uppsala/advisors">Advisors</a></li>
</ul></li>
</ul></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li>
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li>
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/bioart">BioArt</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/LactonutritiousWorld">A LactoWorld</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/killswitches">Killswitches</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/realization">Patent</a></li>
</ul></li>
</ul></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a>
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<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a></li>  
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                                <ul>
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                                    <li><a href="https://2013.igem.org/Team:Uppsala/collaboration">Collaboration</a></li>
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                                </ul></li>  
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<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a>
                                     <ul>
                                     <ul>
                                         <li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li>
                                         <li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li>
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                                        <li><a href="https://2013.igem.org/Team:Uppsala/protocols">Protocols</a></li>
                                     </ul></li>
                                     </ul></li>
</ul>
</ul>
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<div id="main_content">  
<div id="main_content">  
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<h1 class="main-title_parts"> Uppsalas contribution to the iGEM community </h1>
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        <h1 class="main-title">Protocols</h1>
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For this years competition we at iGEM Uppsala 2013 have deposited <b> 63 new biobricks </b> to the registry. Biobricks such as <b> tools for our new Lactobacillus genus </b> introduced to the iGEM community, <b> new basic components </b> for the production of metabolites previously not found in the registry, an <b> improvement of various biobrick parts </b> and last but not least we have also deposited various parts that have never made its way to the registry before. Each and every parts performance has been <b> extensively documented </b> during its use and all of the information will be able to be previewed as an experience on each part.
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<ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2013/e/e7/Uppsala2013_3A_assembly.cloning_cycle.pdf">3A assembly cloning cycle</a></li>      
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<li><a href="https://static.igem.org/mediawiki/2013/e/e7/Uppsala2013_3A_assembly.cloning_cycle.pdf">Agarose gel electrophoresis</a></li>
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<br>
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      <li><a href="https://static.igem.org/mediawiki/2013/2/2c/Uppsala2013_Autoclaver_manual.pdf">Autoclaver manual</a></li>
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<br>
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iGEM Uppsala 2013 has worked hard on <b> improving biobricks </b> such as <b> stilbene synthase </b> <a href="http://parts.igem.org/Part:BBa_K1033002">BBa_K1033002</a> (STS) and <b> 4-coumarate CoA ligase </b> <a href="http://parts.igem.org/Part:BBa_K1033001">BBa_K1033001</a> (4CL) that were worked on by Rice universitys iGEM team 2008. These biobricks will now be <b> available for the first time </b> in the registry containing a complete description of our experiences with the part.
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        <li><a href="https://static.igem.org/mediawiki/2013/1/1c/Uppsala2013_BioBrick_Assembly_Manual.pdf">BioBrick assembly manual</a></li>
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<br>
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        <li><a href="https://static.igem.org/mediawiki/2013/1/1c/Uppsala2013_BioBrick_Assembly_Manual.pdf">CCMB80 buffer protocol</a></li>
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<br>
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Finally iGEM Uppsala are proud to present our <b> complete chromoprotein collection </b>, a collection of reporter genes that are directly visible to the naked eye. All the chromoproteins from iGEM Uppsala team 2011 & 2012 will finally be available through the registry. We have also expanded our collection by <b> introducing 8 new chromoproteins </b> such as meffBlue <a href="http://parts.igem.org/Part:BBa_K1033901"> BBa_K1033901, </a> tsPurple <a href="http://parts.igem.org/Part:BBa_K1033905"> BBa_K1033905, </a> fwYellow <a href="http://parts.igem.org/Part:BBa_K1033909"> BBa_K1033909, </a> scOrange <a href="http://parts.igem.org/Part:BBa_K1033913"> BBa_K1033913, </a> gfasPurple <a href="http://parts.igem.org/Part:BBa_K1033918"> BBa_K1033918, </a> spisPink <a href="http://parts.igem.org/Part:BBa_K1033925"> BBa_K1033925, </a> asPink <a href="http://parts.igem.org/Part:BBa_K1033927"> BBa_K1033927, </a> amajLime <a href="http://parts.igem.org/Part:BBa_K1033915"> BBa_K1033915 </a>
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        <li><a href="https://static.igem.org/mediawiki/2013/1/1c/Uppsala2013_BioBrick_Assembly_Manual.pdf">Competent cell preparation</a></li>
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<br>
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        <li><a href="https://static.igem.org/mediawiki/2013/f/f9/Uppsala2013_Competitiontest.pdf">Competitiontest</a></li>
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<br>
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<h1> BioBricks constructed during iGEM 2013 </h1>
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        <li><a href="https://static.igem.org/mediawiki/2013/2/29/Uppsala2013_High_pressure_liquid_chromatography.pdf">High pressure liquid chromatography</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2013/e/e4/Uppsala2013_LB_and_LB-agar_protocol.pdf">LB and LB-agar protocol</a></li>
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<div id="chassi"> <h3> <u> Chassi </u> </h3>
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        <li><a href="https://static.igem.org/mediawiki/2013/f/fe/Uppsala2013_M17_medium_preparation%2C_Lactococcus_lactis_-_Kopia.pdf">M17 medium preparation, Lactococcus lactis</a></li>
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<a href="http://parts.igem.org/Part:BBa_K1033206">BBa_K1033206</a> - Shuttle vector pSBLbC for e.coli and lactobacillus <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033207">BBa_K1033207</a> - Shuttle vector pSBLbE for E. coli and Lactobacillus <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033219">BBa_K1033219</a> - Promoter CP1 <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033220">BBa_K1033220</a> - Promoter CP8 <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033221">BBa_K1033221</a> - Promoter CP11 <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033222">BBa_K1033222</a> - Promoter CP29 <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033223">BBa_K1033223</a> - Promoter CP30 <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033224">BBa_K1033224</a> - Promoter CP41 <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033225">BBa_K1033225</a> - Promoter CP44 <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033250">BBa_K1033250</a> - mCherry fusion protein codon optimised for Lactobacillus reuteri <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033259">BBa_K1033259</a> - antitoxin system from lactobacillus plantarum Toxin<br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033260">BBa_K1033260</a> - antitoxin system from Lactobacillus plantarum Toxin<br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033280">BBa_K1033280</a> - aeBlue blue chromoprotein with strong promoter <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033281">BBa_K1033281</a> - amilCP blue chromoprotein with medium strength promoter <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033282">BBa_K1033282</a> - amilCP blue chromoprotein with strong promoter <br>
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-
</div>
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        <li><a href="https://static.igem.org/mediawiki/2013/2/25/Uppsala2013_Overnight_culture_and_glycerol_stock.pdf">Overnight culture and glycerol stock</a></li>
-
</div>
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-
         <div id="sub_content_parts">  
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         <li><a href="https://2013.igem.org/File:Phusion_PCR_Blank_protocol.xls">Phusion PCR Blank protocol</a></li>
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<div id="carotenoid"> <h3> <u> Carotenoid </u> </h3>  
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-
<a href="http://parts.igem.org/Part:BBa_K1033100">BBa_K1033100</a> - geranylgeranyl diphosphate synthase (B0034CrtE) <br>
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        <li><a href="https://static.igem.org/mediawiki/2013/c/c1/Uppsala2013_Plasmidstability.pdf">Plasmidstability</a></li>
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<a href="http://parts.igem.org/Part:BBa_K1033103">BBa_K1033103</a> - geranyl diphosphate synthase <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033104">BBa_K1033104</a> - Isopentenyl diphosphate isomerase <br>
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-
</div>
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-
  </div>
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 +
        <li><a href="https://static.igem.org/mediawiki/2013/d/d3/Uppsala2013_Preparation_of_stocks_for_Lactococcus_lactis_-_Kopia.pdf">Preparation of stocks for Lactococcus lactis</a></li>
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<div id="sub_content_parts">  
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        <li><a href="https://static.igem.org/mediawiki/2013/b/b4/Uppsala2013_PreparationOfCEllsWesternblot.pdf">Preparation of cells Westernblot</a></li>
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<div id="resveratrol"> <h3> <u> Resveratrol </u> </h3>  
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-
<a href="http://parts.igem.org/Part:BBa_K1033000">BBa_K1033000</a> - tyrosine ammonia lyase (TAL) with RBS <br>
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        <li><a href="https://static.igem.org/mediawiki/2013/e/ec/Uppsala2013_Spectrophotometry_of_tyrosine_ammonia_lyase.pdf">Spectrophotometry of tyrosine ammonia lyase</a></li>
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<a href="http://parts.igem.org/Part:BBa_K1033001">BBa_K1033001</a> - 4 coumarate ligase (4CL) with RBS <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033002">BBa_K1033002</a> - stilbene synthase (STS) with RBS <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033003">BBa_K1033003</a> - B0034-4Cl-B0034-STS <br></div>
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-
  </div>
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-
  <div id="sub_content_parts">
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        <li><a href="https://static.igem.org/mediawiki/2013/7/7e/Uppsala2013_TBE-buffer_protocol.pdf">TBE-buffer protocol</a></li>
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<div id="saffron"> <h3> <u> Saffron </u> </h3>
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-
<a href="http://parts.igem.org/Part:BBa_K1033110">BBa_K1033110</a> - Zeaxanthin 7,8-cleavage dioxygenase (ZCD) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033111">BBa_K1033111</a> - Zeaxanthin 7,8-cleavage dioxygenase (ZCDHis) with His tag <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033112">BBa_K1033112</a> - "Uracil glucosyltransferase (UGTCs2)" <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033113">BBa_K1033113</a> - Uracil glucosyltransferase (B0034UGTCs2-6xHis) with RBS and Histag <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033115">BBa_K1033115</a> - Histidine Patch Thioredoxin <br>
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-
</h3>
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-
</div>
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-
</div>
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-
<div id="sub_content_parts">
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        <li><a href="https://static.igem.org/mediawiki/2013/8/81/Uppsala2013_Transformation_by_Electroporation_for_L.reuteri.pdf">Transformation by Electroporation for L.reuteri</a></li>
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<div id="sweetner"> <h3> <u> Sweetner (Miraculin) </u> </h3>  
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-
<a href="http://parts.igem.org/Part:BBa_K1033120">BBa_K1033120</a> - B0034-ExpTag-Linker-Miraculin-Linker-6xHis <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033122">BBa_K1033122</a> - B0034-Miraculin <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033121">BBa_K1033121</a> - Miraculin<br>
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-
</div>
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-
</div>
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-
<div id="sub_content_parts">
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        <li><a href="https://static.igem.org/mediawiki/2013/5/59/Uppsala2013_Transforming_e-coli_protocol.pdf">Transforming e-coli protocol</a></li>
-
<div id="chromoprotein_collection"> <h3> <u> Complete Chromoprotein Collection </u> </h3>  
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-
<a href="http://parts.igem.org/Part:BBa_K1033900">BBa_K1033900</a> - meffBlue, blue chromoprotein (incl RBS, J23110) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033901">BBa_K1033901</a> - meffBlue, blue chromoprotein (incl RBS) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033902">BBa_K1033902</a> - meffBlue, blue chromoprotein <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033903">BBa_K1033903</a> - tsPurple, purple chromoprotein (incl RBS, J23110) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033904">BBa_K1033904</a> - tsPurple, purple chromoprotein (incl RBS, J23106) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033905">BBa_K1033905</a> - tsPurple, purple chromoprotein (incl RBS) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033906">BBa_K1033906</a> - tsPurple, purple chromoprotein <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033907">BBa_K1033907</a> - fwYellow, yellow chromoprotein (incl RBS, J23110) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033908">BBa_K1033908</a> - fwYellow, yellow chromoprotein (incl RBS, J23106) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033909">BBa_K1033909</a> - fwYellow, yellow chromoprotein (incl RBS) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033910">BBa_K1033910</a> - fwYellow, yellow chromoprotein <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033911">BBa_K1033911</a> - scOrange, orange chromoprotein (incl RBS, J23110) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033912">BBa_K1033912</a> - scOrange, orange chromoprotein (incl RBS, J23106) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033913">BBa_K1033913</a> - scOrange, orange chromoprotein (incl. RBS) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033914">BBa_K1033914</a> - amajLime, yellow-green chromoprotein (incl RBS & J23110) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033915">BBa_K1033915</a> - amajLime, yellow-green chromoprotein (incl RBS) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033916">BBa_K1033916</a> - amajLime, yellow-green chromoprotein <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033917">BBa_K1033917</a> - gfasPurple, purple chromoprotein (incl RBS, J23110) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033918">BBa_K1033918</a> - gfasPurple, purple chromoprotein (incl RBS) <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033919">BBa_K1033919</a> - gfasPurple, purple chromoprotein <br>
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-
<a href="http://parts.igem.org/Part:BBa_K1033920">BBa_K1033920</a> - meffRed, red chromoprotein (incl RBS, J23110) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033921">BBa_K1033921</a> - meffRed, red chromoprotein (incl RBS, J23106) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033922">BBa_K1033922</a> - meffRed, red chromoprotein (incl RBS) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033923">BBa_K1033923</a> - spisPink, pink chromoprotein (incl RBS, J23110) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033924">BBa_K1033924</a> - spisPink, pink chromoprotein (incl RBS, J23106) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033925">BBa_K1033925</a> - spisPink, pink chromoprotein (incl RBS) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033926">BBa_K1033926</a> - asPink, pink chromoprotein (incl RBS, J23110) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033927">BBa_K1033927</a> - asPink, pink chromoprotein (incl RBS) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033928">BBa_K1033928</a> - aeBlue, blue chromoprotein (incl RBS, J23110) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033929">BBa_K1033929</a> - aeBlue, blue chromoprotein (incl RBS) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033930">BBa_K1033930</a> - amilCP, blue/purple chromoprotein (incl RBS) <br>
+
-
<a href="http://parts.igem.org/Part:BBa_K1033931">BBa_K1033931</a> - amilGFP, yellow chromoprotein (incl RBS) <br>
+
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        <li><a href="https://static.igem.org/mediawiki/2013/2/23/Uppsala2013_WesternBlot.pdf">WesternBlot</a></li>
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<div id="resistance_casette"> <h3> <u> New antibiotic resistance marker for E.coli </u> </h3>  
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<a href="http://parts.igem.org/Part:BBa_K1033990">BBa_K1033990</a> - Zeocin resistance cassette <br>
 
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Latest revision as of 20:02, 27 October 2013

Retrieved from "http://2013.igem.org/Team:Uppsala/protocols"