Team:Colombia Uniandes/How to parts

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<h2><center>How to parts</center></h2>
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{| border="2" align="center"
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|+'''Primers used to construct Nicko and Chimi'''
 +
| style="text-align: center;" |Name
 +
| style="text-align: center;" |Sequence
 +
| style="text-align: center;" |Identification Number
 +
 +
|-
 +
| style="text-align: left;" |FW XbaI-BAP2
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| style="text-align: left;" |CAC CTC TAG AGA GCA TTA ATG CAA GTC GAG AAA GG
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| style="text-align: center;" |19
 +
 +
|-
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| style="text-align: left;" |FW XbaI-GAL1
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| style="text-align: left;" |CAC CTC TAG AGG CTA TAT TGA AGT ACG GAT TAG
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| style="text-align: center;" |15
 +
 +
|-
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| style="text-align: left;" |FW Fusion GAL1-KOZAC-mCherry
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| style="text-align: left;" |GGA AAC TAG ACC CGC CGC CAC CAT GGA GAT GGT GAG CAA GGG CGA GG
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| style="text-align: center;" |17
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 +
|-
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| style="text-align: left;" |RV Fusion GAL1-KOZAC-mCherry
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| style="text-align: left;" |CCT CGC CCT TGC TCA CCA TCT CCA TGG TGG CGG CGG GTC TAG TTT CC
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| style="text-align: center;" |13
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|-
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| style="text-align: left;" |FW Fusion BAP2-KOZAC-GAL4
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| style="text-align: left;" |CTC AAG TGA TTT TAG AAC CCG CCG CCA CCA TGG AGA TGA AGC TAC TGT CTT C
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| style="text-align: center;" |11
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 +
|-
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| style="text-align: left;" |RV Fusion BAP2-KOZAC-GAL4
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| style="text-align: left;" |GAA GAC AGT AGC TTC ATC TCC ATG GTG GCG GCG GGT TCT AAA ATC ACT TGA G
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| style="text-align: center;" |3
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|-
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| style="text-align: left;" |FW FUSION GAL4-VP16-1
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| style="text-align: left;" |CAG TTG ACT GTA TCT TCG AGG TCG ACC ATT CAG ATC TCA CGA GCC GCC CCC CCG ACC G
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| style="text-align: center;" |1
 +
|-
 +
| style="text-align: left;" |RV FUSION GAL4-VP16-1
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| style="text-align: left;" |CGG TCG GGG GGG CGG CTC GTG AGA TCT GAA TGG TCG ACC TCG AAG ATA CAG TCA ACT G
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| style="text-align: center;" |5
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|-
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| style="text-align: left;" |FW FUSION VP6-GCR
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| style="text-align: left;" |CCT ACG GCG CTC TGG ATA TGA TCT CTC GAG CCA TTC AGC AAG CCA CTG CAG G
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| style="text-align: center;" |9
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|-
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| style="text-align: left;" |RV FUSION VP6-GCR
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| style="text-align: left;" |CCT GCA GTG GCT TGC TGA ATG GCT CGA GAG ATC ATA TCC AGA GCG CCG TAG G
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| style="text-align: center;" |6
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|-
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| style="text-align: left;" |FW Fusion GCR-yeastTerminador
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| style="text-align: left;" |GCT TCT GTT TCA TCA AAA ATG AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG
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| style="text-align: center;" |35
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|-
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| style="text-align: left;" |RV Fusion GCR-yeastTerminador
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| style="text-align: left;" |CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTC ATT TTT GAT GAA ACA GAA GC
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| style="text-align: center;" |34
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|-
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| style="text-align: left;" |FW Fusion mCherry-yeastTerminador
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| style="text-align: left;" |GGA CGA GCT GTA CAA GTA ATA AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG
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| style="text-align: center;" |33
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|-
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| style="text-align: left;" |RV Fusion mCherry-yeastTerminador
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| style="text-align: left;" |CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTT ATT ACT TGT ACA GCT CGT CC
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| style="text-align: center;" |32
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|-
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| style="text-align: left;" |FW Constructo final
 +
| style="text-align: left;" |ACC GTC TAG ATT GAC AGC TAG CTC AGT CCT AGG
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| style="text-align: center;" |40
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|-
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| style="text-align: left;" |FW HoxN
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| style="text-align: left;" |ATG GAA GCA CTG CCG AAC GAT T
 +
| style="text-align: center;" |42
 +
|-
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| style="text-align: left;" |RV HoxNconColamRfP
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| style="text-align: left;" |GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG
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| style="text-align: center;" |44
 +
|-
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| style="text-align: left;" |FW mRFPconColaHoxN
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| style="text-align: left;" |GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA
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| style="text-align: center;" |41
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|-
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| style="text-align: left;" |RV mRFPconXba
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| style="text-align: left;" |ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC
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| style="text-align: center;" |38
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|-
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| style="text-align: left;" |RcnR+B1006 RV
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| style="text-align: left;" |AAA AAC CCC GCC CTG TCA GGG GCG GGG TTT TTT TTT TTA TTT GAT ATA TGA ATC CAG CAC
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| style="text-align: center;" |10
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|-
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| style="text-align: left;" |FW Prcna
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| style="text-align: left;" |GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT
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| style="text-align: center;" |43
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|-
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| style="text-align: left;" |RV Prcna
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| style="text-align: left;" |TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT
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| style="text-align: center;" |39
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|-
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| style="text-align: left;" |J23117+B0034+RcnR FW
 +
| style="text-align: left;" |GAC AGC TAG CTC AGT CCT AGG GAT TGT GCT AGC AAA GAG GAG AAA ATG TCT CAT ACA ATC
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| style="text-align: center;" |23
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|-
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| style="text-align: left;" |J23117+B0034+RcnR RV
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| style="text-align: left;" |GAT TGT ATG AGA CAT TTT CTC CTC TTT GCT AGC ACA ATC CCT AGG ACT GAG CTA GCT GTC
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| style="text-align: center;" |21
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|-
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|}
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Latest revision as of 01:29, 26 June 2014

How to parts


Primers used to construct Nicko and Chimi
Name Sequence Identification Number
FW XbaI-BAP2 CAC CTC TAG AGA GCA TTA ATG CAA GTC GAG AAA GG 19
FW XbaI-GAL1 CAC CTC TAG AGG CTA TAT TGA AGT ACG GAT TAG 15
FW Fusion GAL1-KOZAC-mCherry GGA AAC TAG ACC CGC CGC CAC CAT GGA GAT GGT GAG CAA GGG CGA GG 17
RV Fusion GAL1-KOZAC-mCherry CCT CGC CCT TGC TCA CCA TCT CCA TGG TGG CGG CGG GTC TAG TTT CC 13
FW Fusion BAP2-KOZAC-GAL4 CTC AAG TGA TTT TAG AAC CCG CCG CCA CCA TGG AGA TGA AGC TAC TGT CTT C 11
RV Fusion BAP2-KOZAC-GAL4 GAA GAC AGT AGC TTC ATC TCC ATG GTG GCG GCG GGT TCT AAA ATC ACT TGA G 3
FW FUSION GAL4-VP16-1 CAG TTG ACT GTA TCT TCG AGG TCG ACC ATT CAG ATC TCA CGA GCC GCC CCC CCG ACC G 1
RV FUSION GAL4-VP16-1 CGG TCG GGG GGG CGG CTC GTG AGA TCT GAA TGG TCG ACC TCG AAG ATA CAG TCA ACT G 5
FW FUSION VP6-GCR CCT ACG GCG CTC TGG ATA TGA TCT CTC GAG CCA TTC AGC AAG CCA CTG CAG G 9
RV FUSION VP6-GCR CCT GCA GTG GCT TGC TGA ATG GCT CGA GAG ATC ATA TCC AGA GCG CCG TAG G 6
FW Fusion GCR-yeastTerminador GCT TCT GTT TCA TCA AAA ATG AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG 35
RV Fusion GCR-yeastTerminador CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTC ATT TTT GAT GAA ACA GAA GC 34
FW Fusion mCherry-yeastTerminador GGA CGA GCT GTA CAA GTA ATA AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG 33
RV Fusion mCherry-yeastTerminador CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTT ATT ACT TGT ACA GCT CGT CC 32
FW Constructo final ACC GTC TAG ATT GAC AGC TAG CTC AGT CCT AGG 40
FW HoxN ATG GAA GCA CTG CCG AAC GAT T 42
RV HoxNconColamRfP GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG 44
FW mRFPconColaHoxN GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA 41
RV mRFPconXba ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC 38
RcnR+B1006 RV AAA AAC CCC GCC CTG TCA GGG GCG GGG TTT TTT TTT TTA TTT GAT ATA TGA ATC CAG CAC 10
FW Prcna GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT 43
RV Prcna TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT 39
J23117+B0034+RcnR FW GAC AGC TAG CTC AGT CCT AGG GAT TGT GCT AGC AAA GAG GAG AAA ATG TCT CAT ACA ATC 23
J23117+B0034+RcnR RV GAT TGT ATG AGA CAT TTT CTC CTC TTT GCT AGC ACA ATC CCT AGG ACT GAG CTA GCT GTC 21

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