Team:ETH Zurich/Modeling

From 2013.igem.org

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=''Mine Cells''=
=''Mine Cells''=
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[[File:eqnSender.png|500px|thumb|Equation system 1: ODE for Mine Cells.]]
<p>The Mine Cells lead to the synthesis the signalling molecule, by constitutive expression of ''luxI'' gene. To reveal the nature of the cells, a coloured-substrate reaction is triggered upon addition of ''5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide''; given that the glycoside hydrolase ''NagZ'' is expressed constitutively. <br><br> The ODEs for the states involved in the sender module are given below:
<p>The Mine Cells lead to the synthesis the signalling molecule, by constitutive expression of ''luxI'' gene. To reveal the nature of the cells, a coloured-substrate reaction is triggered upon addition of ''5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide''; given that the glycoside hydrolase ''NagZ'' is expressed constitutively. <br><br> The ODEs for the states involved in the sender module are given below:
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[[File:eqnSender.png|500px|center|thumb|Equation system 1: ODE for Mine Cells.]]
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= ''Biosensors'' =
= ''Biosensors'' =
hg
hg

Revision as of 15:50, 17 August 2013

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The change of the species concentrations in time is given by non-linear ordinary differential equations (ODEs), most of which follow Hill kinetics. The parameters we used in the model are derived from literature.

Mine Cells

Equation system 1: ODE for Mine Cells.

The Mine Cells lead to the synthesis the signalling molecule, by constitutive expression of luxI gene. To reveal the nature of the cells, a coloured-substrate reaction is triggered upon addition of 5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide; given that the glycoside hydrolase NagZ is expressed constitutively.

The ODEs for the states involved in the sender module are given below:


Biosensors

hg