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Team:Paris Saclay/Notebook/July/10
From 2013.igem.org
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For regulation system: | For regulation system: | ||
| - | *prepared the solution of BioBrick fnr repressor in PsB1C3 plasmid for DNA sequencing | + | *prepared the solution of BioBrick fnr repressor in PsB1C3 plasmid for DNA sequencing. |
| - | *the terminator transformation of yesterday did not work, a second transformation had | + | *the terminator transformation of yesterday did not work, a second transformation had been done for it. |
| - | been done for it | + | *The transformation for RBS+LacZ+terminator plasmid into competent cells was performed. |
| - | *The transformation for RBS+LacZ+terminator plasmid into competent cells was | + | *after the transformation PSB3K3 plasmid in competent cells, these cells were cultured in a liquid nutritive medium. |
| - | performed. | + | |
| - | *after the transformation PSB3K3 plasmid in competent cells, these cells were cultured in a | + | |
| - | liquid nutritive medium | + | |
<br> | <br> | ||
For PSBs sensor system: | For PSBs sensor system: | ||
*the ligation products were transformed into competent cells and were cultured on solid | *the ligation products were transformed into competent cells and were cultured on solid | ||
| - | medium with their specific antibiotics | + | medium with their specific antibiotics. |
=='''Lab work'''== | =='''Lab work'''== | ||
Revision as of 03:02, 28 August 2013
Notebook : July 10
Summary:
For regulation system:
- prepared the solution of BioBrick fnr repressor in PsB1C3 plasmid for DNA sequencing.
- the terminator transformation of yesterday did not work, a second transformation had been done for it.
- The transformation for RBS+LacZ+terminator plasmid into competent cells was performed.
- after the transformation PSB3K3 plasmid in competent cells, these cells were cultured in a liquid nutritive medium.
For PSBs sensor system:
- the ligation products were transformed into competent cells and were cultured on solid
medium with their specific antibiotics.
Lab work
constructing
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