Team:ETH Zurich/Attributions

From 2013.igem.org

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<h1>Contributions</h1>
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<p>The work in the lab as well as the modeling part was fulfilled by the team members only. <br>We want to thanks our Advisors for supporting the project design, result interpretation and trouble shooting.</p>
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<p>The experimental work in the lab, computational modeling and the webpage was done by team members only. Every student had a responsibility for a particular task but of course work in the lab as well as on the wiki. Meret was responsible for the human practice, optimization and cloning, Parvathi for the proof of principle experiments with GFP, Angela for the hydrolase tests, Matthias for the promoter library, Susanna for the Model and Fabian for the WIKI.<br>We thank our advisors for their continuous support in result interpretation, trouble shooting and debugging of the experiments, model and the WIKI.</p>
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<h1><b>Acknowledgement</b></h1>
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<h1>Acknowledgement</h1>
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Thanks to <i>Dr. Johannes Haerle</i> (from Panke Group, D-BSSE Basel) for the hydrolase parts<br>
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<p>Thanks to <i>Dr. Johannes Haerle</i> (PostDoc, Panke Group D-BSSE Basel) for the hydrolases<br><br>
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Thanks to <i>Anke Gehringer</i> (Technical Assistent, Panke Group D-BSSE Basel) for the enzyme kinetics protocols<br><br>
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Thanks to <i>Andreas Bosshart</i> (PhD student, Panke Group D-BSSE Basel) for the plasmid backbones<br><br>
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Thanks to <i>Niels Bürckert</i> (BSO, D-BSSE Basel) for helpful discussions concerning the safety form<br><br>
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Thanks to <i>Verena Jaeggin</i> and the whole <i>Single cell facility group</i> for support concerning the Fortessa FACS analyser and the iTecan infinity M200 plate reader<br><br>
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Thanks to <i>Dr. Thomas Horn</i> for taking time from his busy schedule to arrange for the microscopy experiment for us.<br><br>
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Thanks to <i>Urs Senn </i> (Technical Assistent, Stelling Group D-BSSE Basel) for the robotic support<br><br>
Thanks to the <i>iGEM 2012 Team ETH Zurich</i> for the general structure of the template header code<br>
Thanks to the <i>iGEM 2012 Team ETH Zurich</i> for the general structure of the template header code<br>
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<br>
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We are extremely grateful to D-BSSE, ETH-Zürich, for their cordial support and guidance for the conduction of our project.
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<h1><b>References</b></h1>
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</p>
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<b>Quorum sensing</b>
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<p>Hai-Bao Zhang, Lian-Hui Wang, Lian-Hui Zhang, 1 JUN 2007 ; DOI: 10.1002/9780471729259.mc01c03s05 <br>UNIT 1C.3 Detection and Analysis of Quorum-Quenching Enzymes Against Acyl Homoserine Lactone Quorum-Sensing Signals <br>[http://onlinelibrary.wiley.com/doi/10.1002/9780471729259.mc01c03s05/full  Wiley Online library]</p>
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<p>Ravn L, Christensen AB, Molin S, Givskov M, Gram L. J Microbiol Methods ; 2001 Apr;44(3):239-51<br>
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Methods for detecting acylated homoserine lactones produced by Gram-negative bacteria and their application in studies of AHL-production kinetics <br> [http://www.ncbi.nlm.nih.gov/pubmed/11240047  PubMed]</p>
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<p>Lingchong You, Robert Sidney Cox, Ron Weiss3 & Frances H. Arnold; Nature 428, 868-871<br>Programmed population control by cell–cell communication and regulated killing<br> [http://www.nature.com/nature/journal/v428/n6985/abs/nature02491.html Nature]</p>
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<p>Ramiz Daniel1 Jacob R. Rubens Rahul Sarpeshkar & Timothy K. Lu;doi:10.1038/nature12148<br>
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Synthetic analog computation in living cells<br>[http://www.nature.com/nature/journal/vaop/ncurrent/full/nature12148.html Nature]</p>
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<p>Subhayu Basu, Yoram Gerchman, Cynthia H. Collins, Frances H. Arnold & Ron Weiss;Nature 434, 1130-1134 (28 April 2005)<br> A synthetic multicellular system for programmed pattern formation <br>[http://www.nature.com/nature/journal/v434/n7037/abs/nature03461.html Nature]</p>
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<p>Grant D. Geske†, Margrith E. Mattmann†, Helen E. Blackwell;Bioorganic & Medicinal Chemistry Letters
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Volume 18, Issue 22, 15 November 2008, Pages 5978–5981<br>
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Evaluation of a focused library of N-aryl l-homoserine lactones reveals a new set of potent quorum sensing modulators
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<br>[http://www.sciencedirect.com/science/article/pii/S0960894X08008652link Science direct]</p>
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<b>Hydrolases</b>
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<p>J. C. Jokerst,J. A. Adkins,B. Bisha,M. M. Mentele, L. D. Goodridge and C. S. Henry ;  Chem. 2012, 84, 2900−2907<br>
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Development of a Paper-Based Analytical Device for Colorimetric, Detection of Select Foodborne Pathogens <br>[http://pubs.acs.org/doi/pdf/10.1021/ac203466y  pubs.acs]</p>
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<p>Choi JH, Jeong KJ, Kim SC, Lee SY; Appl Microbiol Biotechnol. ; 2000 Jun;53(6):640-5.<br>
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Efficient secretory production of alkaline phosphatase by high cell density culture of recombinant Escherichia coli using the Bacillus sp. endoxylanase signal sequence.<br>[http://www.ncbi.nlm.nih.gov/pubmed/10919319 PubMed]</p>
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<p> Kiernan JA; Biotech Histochem. 2007 Apr; 82(2):73-103. <br>
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Indigogenic substrates for detection and localization of enzymes. <br> [http://www.ncbi.nlm.nih.gov/pubmed/17577701 PubMed]</p>
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<p> Jefferson RA; US 5432081 A. 1995 Jul <br>
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Host cells transformed with the ''E. coli'' glucoronide permease gene </p>
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Latest revision as of 12:55, 28 October 2013

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Contributions

The experimental work in the lab, computational modeling and the webpage was done by team members only. Every student had a responsibility for a particular task but of course work in the lab as well as on the wiki. Meret was responsible for the human practice, optimization and cloning, Parvathi for the proof of principle experiments with GFP, Angela for the hydrolase tests, Matthias for the promoter library, Susanna for the Model and Fabian for the WIKI.
We thank our advisors for their continuous support in result interpretation, trouble shooting and debugging of the experiments, model and the WIKI.

Acknowledgement

Thanks to Dr. Johannes Haerle (PostDoc, Panke Group D-BSSE Basel) for the hydrolases

Thanks to Anke Gehringer (Technical Assistent, Panke Group D-BSSE Basel) for the enzyme kinetics protocols

Thanks to Andreas Bosshart (PhD student, Panke Group D-BSSE Basel) for the plasmid backbones

Thanks to Niels Bürckert (BSO, D-BSSE Basel) for helpful discussions concerning the safety form

Thanks to Verena Jaeggin and the whole Single cell facility group for support concerning the Fortessa FACS analyser and the iTecan infinity M200 plate reader

Thanks to Dr. Thomas Horn for taking time from his busy schedule to arrange for the microscopy experiment for us.

Thanks to Urs Senn (Technical Assistent, Stelling Group D-BSSE Basel) for the robotic support

Thanks to the iGEM 2012 Team ETH Zurich for the general structure of the template header code
We are extremely grateful to D-BSSE, ETH-Zürich, for their cordial support and guidance for the conduction of our project.


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