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Team:Calgary/Sandbox/Notebook/Protocols
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<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/KapaPCR" target="_blank">Kapa PCR</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/KapaPCR" target="_blank">Kapa PCR</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/PCRPurification" target="_blank">PCR Purification</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/PCRPurification" target="_blank">PCR Purification</a></li> | ||
| - | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/PlasmidPurificationfromEcoli" target="_blank">Plasmid Purification from E. coli</a></li> | + | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/PlasmidPurificationfromEcoli" target="_blank">Plasmid Purification from <i>E. coli</i></a></li> |
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/RehydrationOfRegistryDNA" target="_blank">Rehydration of Registry DNA</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/RehydrationOfRegistryDNA" target="_blank">Rehydration of Registry DNA</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/RestrictionDigestionAntarcticPhosphataseAndLigation" target="_blank">Restriction Digestion, Antarctic Phosphatase, and Ligation</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/RestrictionDigestionAntarcticPhosphataseAndLigation" target="_blank">Restriction Digestion, Antarctic Phosphatase, and Ligation</a></li> | ||
| Line 28: | Line 28: | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/OsmoticShock" target="_blank">Osmotic Shock</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/SDSPAGEGel" target="_blank">SDS PAGE Gel</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/SDSPAGEGel" target="_blank">SDS PAGE Gel</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/WesternBlot" target="_blank">Western Blot</a></li> | ||
</ul> | </ul> | ||
<h2>TALE Protocols</h2> | <h2>TALE Protocols</h2> | ||
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<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GelShiftAssay" target="_blank">Gel Shift Assay</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GelShiftAssay" target="_blank">Gel Shift Assay</a></li> | ||
</ul> | </ul> | ||
| - | <h2> | + | <h2>Ferritin Protocols</h2> |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/Kinetics" target="_blank">Kinetics</a></li> |
</ul> | </ul> | ||
</section> | </section> | ||
</html> | </html> | ||
Latest revision as of 23:44, 22 September 2013
Protocols
General Protocols
- Bacterial Transformation
- Glycerol Stocks
- LB Agar Plates
- Making Chemically Competent E. coli Cells
- Overnight Cultures
DNA Protocols
- Agarose Gel Electrophoresis
- Colony PCR
- Gel Extraction
- Kapa PCR
- PCR Purification
- Plasmid Purification from E. coli
- Rehydration of Registry DNA
- Restriction Digestion, Antarctic Phosphatase, and Ligation
- Verification Digest
Protein Protocols
- Bradford Assay
- Freeze-Thaw Protocol for Protein Samples
- Glass Beads Cell Lysis Protocol for Protein Samples
- Osmotic Shock
- SDS PAGE Gel
- Western Blot
