Team:Colombia Uniandes/How to parts

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**Primers used to construct Nicko and Chimi**
Name	Sequence	Identification Number
FW XbaI-BAP2	CAC CTC TAG AGA GCA TTA ATG CAA GTC GAG AAA GG	19
FW XbaI-GAL1	CAC CTC TAG AGG CTA TAT TGA AGT ACG GAT TAG	15
FW Fusion GAL1-KOZAC-mCherry	GGA AAC TAG ACC CGC CGC CAC CAT GGA GAT GGT GAG CAA GGG CGA GG	17
RV Fusion GAL1-KOZAC-mCherry	CCT CGC CCT TGC TCA CCA TCT CCA TGG TGG CGG CGG GTC TAG TTT CC	13
FW Fusion BAP2-KOZAC-GAL4	CTC AAG TGA TTT TAG AAC CCG CCG CCA CCA TGG AGA TGA AGC TAC TGT CTT C	11
RV Fusion BAP2-KOZAC-GAL4	GAA GAC AGT AGC TTC ATC TCC ATG GTG GCG GCG GGT TCT AAA ATC ACT TGA G	3
FW FUSION GAL4-VP16-1	CAG TTG ACT GTA TCT TCG AGG TCG ACC ATT CAG ATC TCA CGA GCC GCC CCC CCG ACC G	1
RV FUSION GAL4-VP16-1	CGG TCG GGG GGG CGG CTC GTG AGA TCT GAA TGG TCG ACC TCG AAG ATA CAG TCA ACT G	5
FW FUSION VP6-GCR	CCT ACG GCG CTC TGG ATA TGA TCT CTC GAG CCA TTC AGC AAG CCA CTG CAG G	9
RV FUSION VP6-GCR	CCT GCA GTG GCT TGC TGA ATG GCT CGA GAG ATC ATA TCC AGA GCG CCG TAG G	6
FW Fusion GCR-yeastTerminador	GCT TCT GTT TCA TCA AAA ATG AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG	35
RV Fusion GCR-yeastTerminador	CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTC ATT TTT GAT GAA ACA GAA GC	34
FW Fusion mCherry-yeastTerminador	GGA CGA GCT GTA CAA GTA ATA AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG	33
RV Fusion mCherry-yeastTerminador	CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTT ATT ACT TGT ACA GCT CGT CC	32
FW Constructo final	ACC GTC TAG ATT GAC AGC TAG CTC AGT CCT AGG	40
FW HoxN	ATG GAA GCA CTG CCG AAC GAT T	42
RV HoxNconColamRfP	GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG	44
FW mRFPconColaHoxN	GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA	41
RV mRFPconXba	ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC	38
RcnR+B1006 RV	AAA AAC CCC GCC CTG TCA GGG GCG GGG TTT TTT TTT TTA TTT GAT ATA TGA ATC CAG CAC	10
FW Prcna	GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT	43
RV Prcna	TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT	39
J23117+B0034+RcnR FW	GAC AGC TAG CTC AGT CCT AGG GAT TGT GCT AGC AAA GAG GAG AAA ATG TCT CAT ACA ATC	23
J23117+B0034+RcnR RV	GAT TGT ATG AGA CAT TTT CTC CTC TTT GCT AGC ACA ATC CCT AGG ACT GAG CTA GCT GTC	21

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-|+'''Primers used to construct Nicko & Chimi'''
+|+'''Primers used to construct Nicko and Chimi'''
 | style="text-align: center;" |Name
 | style="text-align: center;" |Sequence
@@ Line 7: / Line 14: @@
 |-
-| style="text-align: center;" |FW XbaI-BAP2
+| style="text-align: left;" |FW XbaI-BAP2
-| style="text-align: left;" |CACCTCTAGAGAGCATTAATGCAAGTCGAGAAAGG
+| style="text-align: left;" |CAC CTC TAG AGA GCA TTA ATG CAA GTC GAG AAA GG
 | style="text-align: center;" |19
 |-
-| style="text-align: center;" |FW XbaI-GAL1
+| style="text-align: left;" |FW XbaI-GAL1
-| style="text-align: left;" |CACCTCTAGAGGCTATATTGAAGTACGGATTAG
+| style="text-align: left;" |CAC CTC TAG AGG CTA TAT TGA AGT ACG GAT TAG
 | style="text-align: center;" |15
 |-
-| style="text-align: center;" |FW Fusion GAL1-KOZAC-mCherry
+| style="text-align: left;" |FW Fusion GAL1-KOZAC-mCherry
-| style="text-align: center;" |GGAAACTAGACCCGCCGCCACCATGGAGATGGTGAGCAAGGGCGAGG
+| style="text-align: left;" |GGA AAC TAG ACC CGC CGC CAC CAT GGA GAT GGT GAG CAA GGG CGA GG
 | style="text-align: center;" |17
 |-
-| style="text-align: center;" |RV Fusion GAL1-KOZAC-mCherry
+| style="text-align: left;" |RV Fusion GAL1-KOZAC-mCherry
-| style="text-align: center;" |CCTCGCCCTTGCTCACCATCTCCATGGTGGCGGCGGGTCTAGTTTCC
+| style="text-align: left;" |CCT CGC CCT TGC TCA CCA TCT CCA TGG TGG CGG CGG GTC TAG TTT CC
 | style="text-align: center;" |13
 |-
-| style="text-align: center;" |FW Fusion BAP2-KOZAC-GAL4
+| style="text-align: left;" |FW Fusion BAP2-KOZAC-GAL4
-| style="text-align: center;" |CTCAAGTGATTTTAGAACCCGCCGCCACCATGGAGATGAAGCTACTGTCTTC
+| style="text-align: left;" |CTC AAG TGA TTT TAG AAC CCG CCG CCA CCA TGG AGA TGA AGC TAC TGT CTT C
 | style="text-align: center;" |11
 |-
-| style="text-align: center;" |RV Fusion BAP2-KOZAC-GAL4
+| style="text-align: left;" |RV Fusion BAP2-KOZAC-GAL4
-| style="text-align: center;" |GAAGACAGTAGCTTCATCTCCATGGTGGCGGCGGGTTCTAAAATCACTTGAG
+| style="text-align: left;" |GAA GAC AGT AGC TTC ATC TCC ATG GTG GCG GCG GGT TCT AAA ATC ACT TGA G
 | style="text-align: center;" |3
 |-
-| style="text-align: center;" |FW FUSION GAL4-VP16-1
+| style="text-align: left;" |FW FUSION GAL4-VP16-1
-| style="text-align: center;" |CAGTTGACTGTATCTTCGAGGTCGACCATTCAGATCTCACGAGCCGCCCCCCCGACCG
+| style="text-align: left;" |CAG TTG ACT GTA TCT TCG AGG TCG ACC ATT CAG ATC TCA CGA GCC GCC CCC CCG ACC G
 | style="text-align: center;" |1
 |-
-| style="text-align: center;" |RV FUSION GAL4-VP16-1
+| style="text-align: left;" |RV FUSION GAL4-VP16-1
-| style="text-align: center;" |CGGTCGGGGGGGCGGCTCGTGAGATCTGAATGGTCGACCTCGAAGATACAGTCAACTG
+| style="text-align: left;" |CGG TCG GGG GGG CGG CTC GTG AGA TCT GAA TGG TCG ACC TCG AAG ATA CAG TCA ACT G
 | style="text-align: center;" |5
 |-
-| style="text-align: center;" |FW FUSION VP6-GCR
+| style="text-align: left;" |FW FUSION VP6-GCR
-| style="text-align: center;" |CCTACGGCGCTCTGGATATGATCTCTCGAGCCATTCAGCAAGCCACTGCAGG
+| style="text-align: left;" |CCT ACG GCG CTC TGG ATA TGA TCT CTC GAG CCA TTC AGC AAG CCA CTG CAG G
 | style="text-align: center;" |9
 |-
-| style="text-align: center;" |RV FUSION VP6-GCR
+| style="text-align: left;" |RV FUSION VP6-GCR
-| style="text-align: center;" |CCTGCAGTGGCTTGCTGAATGGCTCGAGAGATCATATCCAGAGCGCCGTAGG
+| style="text-align: left;" |CCT GCA GTG GCT TGC TGA ATG GCT CGA GAG ATC ATA TCC AGA GCG CCG TAG G
 | style="text-align: center;" |6
 |-
-| style="text-align: center;" |FW Fusion GCR-yeastTerminador
+| style="text-align: left;" |FW Fusion GCR-yeastTerminador
-| style="text-align: center;" |GCTTCTGTTTCATCAAAAATGAAAAGTGAATATCCAAACGAGAATGTCAATGG
+| style="text-align: left;" |GCT TCT GTT TCA TCA AAA ATG AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG
 | style="text-align: center;" |35
 |-
-| style="text-align: center;" |RV Fusion GCR-yeastTerminador
+| style="text-align: left;" |RV Fusion GCR-yeastTerminador
-| style="text-align: center;" |CCATTGACATTCTCGTTTGGATATTCACTTTTCATTTTTGATGAAACAGAAGC
+| style="text-align: left;" |CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTC ATT TTT GAT GAA ACA GAA GC
 | style="text-align: center;" |34
 |-
-| style="text-align: center;" |FW Fusion mCherry-yeastTerminador
+| style="text-align: left;" |FW Fusion mCherry-yeastTerminador
-| style="text-align: center;" |GGACGAGCTGTACAAGTAATAAAAAGTGAATATCCAAACGAGAATGTCAATGG
+| style="text-align: left;" |GGA CGA GCT GTA CAA GTA ATA AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG
 | style="text-align: center;" |33
 |-
-| style="text-align: center;" |RV Fusion mCherry-yeastTerminador
+| style="text-align: left;" |RV Fusion mCherry-yeastTerminador
-| style="text-align: center;" |CCATTGACATTCTCGTTTGGATATTCACTTTTTATTACTTGTACAGCTCGTCC
+| style="text-align: left;" |CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTT ATT ACT TGT ACA GCT CGT CC
 | style="text-align: center;" |32
 |-
-| style="text-align: center;" |FW HoxN
+| style="text-align: left;" |FW Constructo final
-| style="text-align: center;" |ATGGAAGCACTGCCGAACGATT
+| style="text-align: left;" |ACC GTC TAG ATT GAC AGC TAG CTC AGT CCT AGG
+| style="text-align: center;" |40
+|-
+| style="text-align: left;" |FW HoxN
+| style="text-align: left;" |ATG GAA GCA CTG CCG AAC GAT T
 | style="text-align: center;" |42
 |-
-| style="text-align: center;" |RV HoxNconColamRfP
+| style="text-align: left;" |RV HoxNconColamRfP
-| style="text-align: center;" |GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG
+| style="text-align: left;" |GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG
 | style="text-align: center;" |44
 |-
-| style="text-align: center;" |FW mRFPconColaHoxN
+| style="text-align: left;" |FW mRFPconColaHoxN
-| style="text-align: center;" |GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA
+| style="text-align: left;" |GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA
 | style="text-align: center;" |41
 |-
-| style="text-align: center;" |RV mRFPconXba
+| style="text-align: left;" |RV mRFPconXba
-| style="text-align: center;" |ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC
+| style="text-align: left;" |ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC
 | style="text-align: center;" |38
 |-
-| style="text-align: center;" |FW Prcna
+| style="text-align: left;" |RcnR+B1006 RV
-| style="text-align: center;" |GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT
+| style="text-align: left;" |AAA AAC CCC GCC CTG TCA GGG GCG GGG TTT TTT TTT TTA TTT GAT ATA TGA ATC CAG CAC
+| style="text-align: center;" |10
+|-
+| style="text-align: left;" |FW Prcna
+| style="text-align: left;" |GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT
 | style="text-align: center;" |43
 |-
-| style="text-align: center;" |RV Prcna
+| style="text-align: left;" |RV Prcna
-| style="text-align: center;" |TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT
+| style="text-align: left;" |TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT
 | style="text-align: center;" |39
 |-
-| style="text-align: center;" |J23117+B0034+RcnR FW
+| style="text-align: left;" |J23117+B0034+RcnR FW
-| style="text-align: center;" |GACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCAAAGAGGAGAAAATGTCTCATACAATC
+| style="text-align: left;" |GAC AGC TAG CTC AGT CCT AGG GAT TGT GCT AGC AAA GAG GAG AAA ATG TCT CAT ACA ATC
 | style="text-align: center;" |23
 |-
-| style="text-align: center;" |J23117+B0034+RcnR RV
+| style="text-align: left;" |J23117+B0034+RcnR RV
-| style="text-align: center;" |GATTGTATGAGACATTTTCTCCTCTTTGCTAGCACAATCCCTAGGACTGAGCTAGCTGTC
+| style="text-align: left;" |GAT TGT ATG AGA CAT TTT CTC CTC TTT GCT AGC ACA ATC CCT AGG ACT GAG CTA GCT GTC
 | style="text-align: center;" |21
 |-
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