"
Team:Paris Saclay/Notebook/July/8
From 2013.igem.org
(Difference between revisions)
(→2 - Electrophoresis of the digestion of BBa_K592009, BBa_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI) |
|||
| (One intermediate revision not shown) | |||
| Line 7: | Line 7: | ||
==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : | + | ===='''Objective : BBa_K1155003, BBa_K1155007'''==== |
| - | ===='''1 - Digestion of | + | ===='''1 - Digestion of BBa_K592009, BBa_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI'''==== |
Abou, Anaïs, Sheng, Zhou | Abou, Anaïs, Sheng, Zhou | ||
| Line 16: | Line 16: | ||
* NotI : | * NotI : | ||
| - | ** | + | ** BBa_K592009 : 2µL |
** Buffer orange : 2µL | ** Buffer orange : 2µL | ||
** NotI : 0.5µL | ** NotI : 0.5µL | ||
| Line 22: | Line 22: | ||
* XhoI : | * XhoI : | ||
| - | ** | + | ** BBa_K592009 : 2µL |
** Buffer red : 2µL | ** Buffer red : 2µL | ||
** XhoI : 0.5µL | ** XhoI : 0.5µL | ||
| Line 28: | Line 28: | ||
* EcoRI : | * EcoRI : | ||
| - | ** | + | ** BBa_K592009 : 2µL |
** Buffer orange : 2µL | ** Buffer orange : 2µL | ||
** EcoRI : 0.5µL | ** EcoRI : 0.5µL | ||
| Line 34: | Line 34: | ||
* EcoRI/PstI : | * EcoRI/PstI : | ||
| - | ** | + | ** BBa_I732017 : 2µL |
** Buffer orange : 2µL | ** Buffer orange : 2µL | ||
** EcoRI : 0.5µL | ** EcoRI : 0.5µL | ||
| Line 42: | Line 42: | ||
We let our digestion 1h30 at 37°C. | We let our digestion 1h30 at 37°C. | ||
| - | ===='''2 - Electrophoresis of the digestion of | + | ===='''2 - Electrophoresis of the digestion of BBa_K592009, BBa_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI'''==== |
Abdou, Anaïs, Sheng, Zhou | Abdou, Anaïs, Sheng, Zhou | ||
| Line 50: | Line 50: | ||
| style="width:350px;border:1px solid black;vertical-align:top;" | | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
* Well 1 : 6µL DNA Ladder | * Well 1 : 6µL DNA Ladder | ||
| - | * Well 2 : 5µL | + | * Well 2 : 5µL BBa_K592009 clone 1 digested by EcoRI+1µL of 6X loading dye |
| - | * Well 3 : 5µL | + | * Well 3 : 5µL BBa_K592009 clone 2 digested by EcoRI+1µL of 6X loading dye |
| - | * Well 4 : 5µL | + | * Well 4 : 5µL BBa_K592009 clone 1 digested by NotI+1µL of 6X loading dye |
| - | * Well 5 : 5µL | + | * Well 5 : 5µL BBa_K592009 clone 2 digested by NotI+1µL of 6X loading dye |
| - | * Well 6 : 5µL | + | * Well 6 : 5µL BBa_K592009 clone 1 digested by XhoI+1µL of 6X loading dye |
| - | * Well 7 : 5µL | + | * Well 7 : 5µL BBa_K592009 clone 2 digested by XhoI+1µL of 6X loading dye |
| - | * Well 8 : 5µL | + | * Well 8 : 5µL BBa_I732017 clone 1 digested by EcoRI/PstI+1µL of 6X loading dye |
| - | * Well 9 : 5µL | + | * Well 9 : 5µL BBa_I732017 clone 2 digested by EcoRI/PstI+1µL of 6X loading dye |
* Well 10 : - | * Well 10 : - | ||
* Well 11 : - | * Well 11 : - | ||
| Line 65: | Line 65: | ||
Expected sizes : | Expected sizes : | ||
| - | * | + | * BBa_K592009 digested by NotI : 2046bp + 693bp |
| - | * | + | * BBa_K592009 digested by XhoI : 1842bp + 892bp |
| - | * | + | * BBa_I732017 : 3093bp |
{| | {| | ||
Latest revision as of 23:55, 4 October 2013
Contents |
Notebook : July 8
Lab work
A - Aerobic/Anaerobic regulation system
Objective : BBa_K1155003, BBa_K1155007
1 - Digestion of BBa_K592009, BBa_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
Abou, Anaïs, Sheng, Zhou
Used quantities :
- NotI :
- BBa_K592009 : 2µL
- Buffer orange : 2µL
- NotI : 0.5µL
- H2O : 15.5µL
- XhoI :
- BBa_K592009 : 2µL
- Buffer red : 2µL
- XhoI : 0.5µL
- H2O : 15.5µL
- EcoRI :
- BBa_K592009 : 2µL
- Buffer orange : 2µL
- EcoRI : 0.5µL
- H2O : 15.5µL
- EcoRI/PstI :
- BBa_I732017 : 2µL
- Buffer orange : 2µL
- EcoRI : 0.5µL
- PstI : 0.5µL
- H2O : 15µL
We let our digestion 1h30 at 37°C.
2 - Electrophoresis of the digestion of BBa_K592009, BBa_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
Abdou, Anaïs, Sheng, Zhou
|
|
Expected sizes :
- BBa_K592009 digested by NotI : 2046bp + 693bp
- BBa_K592009 digested by XhoI : 1842bp + 892bp
- BBa_I732017 : 3093bp
|
We obtain fragments at the right size. Our extraction was good. |
| Previous week | Back to calendar | Next day |
