"
Team:Paris Saclay/Notebook/July/10
From 2013.igem.org
(Difference between revisions)
(→1 - Transformation of ligation of PSB1C3 and BphA1 or BphR1 or BphR2) |
|||
| Line 35: | Line 35: | ||
===='''Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein'''==== | ===='''Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein'''==== | ||
| - | ===='''1 - Transformation of ligation of | + | ===='''1 - Transformation of ligation of pSB1C3 and BphA1 or BphR1 or BphR2'''==== |
Abdou | Abdou | ||
Latest revision as of 16:36, 4 October 2013
Contents |
Notebook : July 10
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155003, BBa_K1155007
1 - Transformation of BBa_I732019 in DH5α
Sheng
Protocol : Bacterial transformation
Objective : obtaining biobricks in pSB3K3
1 - Mini and maxi preparation of the culture of BBa_J04450
Abdou
|
Transformation of BBa_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations. |
Protocol : Mini and maxi preparation
We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
B - PCB sensor system
Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein
1 - Transformation of ligation of pSB1C3 and BphA1 or BphR1 or BphR2
Abdou
Protocol : Bacterial transformation
| Previous day | Back to calendar | Next day |
