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Team:Uppsala/protocols
From 2013.igem.org
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| - | {{Team:Uppsala/standard-style}} | + | {{Team:Uppsala/standard-style-protocols}} |
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<div id="igem"><a href="https://2013.igem.org/Main_Page"><img class="igem" src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_Uppsala_IGEM_log_blue.png"></a></div> | <div id="igem"><a href="https://2013.igem.org/Main_Page"><img class="igem" src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_Uppsala_IGEM_log_blue.png"></a></div> | ||
| + | <img class="slogan" src="https://static.igem.org/mediawiki/2013/a/ad/Uppsala2013_Header.png"> | ||
</div> | </div> | ||
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<li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a> | <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid"> | + | <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">p-Coumaric acid</a></li> |
<li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li> | ||
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<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a> | <li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway"> | + | <li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">Kinetic model</a></li> |
<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li> | ||
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| + | <li><a href="https://2013.igem.org/Team:Uppsala/toxicity-model">Toxicity model</a></li> | ||
</ul></li> | </ul></li> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li> | ||
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<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Uppsala/advisors">Advisors</a></li> | ||
</ul></li> | </ul></li> | ||
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<li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li> | ||
| - | + | <li><a href="https://2013.igem.org/Team:Uppsala/bioart">BioArt</a></li> | |
| + | <li><a href="https://2013.igem.org/Team:Uppsala/LactonutritiousWorld">A LactoWorld</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Uppsala/killswitches">Killswitches</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Uppsala/realization">Patent</a></li> | ||
</ul></li> | </ul></li> | ||
| - | <li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a | + | <li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a></li> |
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<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a> | <li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a> | ||
<ul> | <ul> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Uppsala/protocols">Protocols</a></li> | ||
</ul></li> | </ul></li> | ||
</ul> | </ul> | ||
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<h1 class="main-title">Protocols</h1> | <h1 class="main-title">Protocols</h1> | ||
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| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2013/e/e7/Uppsala2013_3A_assembly.cloning_cycle.pdf">3A assembly cloning cycle</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/e/e7/Uppsala2013_3A_assembly.cloning_cycle.pdf">Agarose gel electrophoresis</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/2/2c/Uppsala2013_Autoclaver_manual.pdf">Autoclaver manual</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/1/1c/Uppsala2013_BioBrick_Assembly_Manual.pdf">BioBrick assembly manual</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/1/1c/Uppsala2013_BioBrick_Assembly_Manual.pdf">CCMB80 buffer protocol</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/1/1c/Uppsala2013_BioBrick_Assembly_Manual.pdf">Competent cell preparation</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/f/f9/Uppsala2013_Competitiontest.pdf">Competitiontest</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/2/29/Uppsala2013_High_pressure_liquid_chromatography.pdf">High pressure liquid chromatography</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/e/e4/Uppsala2013_LB_and_LB-agar_protocol.pdf">LB and LB-agar protocol</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/f/fe/Uppsala2013_M17_medium_preparation%2C_Lactococcus_lactis_-_Kopia.pdf">M17 medium preparation, Lactococcus lactis</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/2/25/Uppsala2013_Overnight_culture_and_glycerol_stock.pdf">Overnight culture and glycerol stock</a></li> | ||
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| + | <li><a href="https://2013.igem.org/File:Phusion_PCR_Blank_protocol.xls">Phusion PCR Blank protocol</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/c/c1/Uppsala2013_Plasmidstability.pdf">Plasmidstability</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/d/d3/Uppsala2013_Preparation_of_stocks_for_Lactococcus_lactis_-_Kopia.pdf">Preparation of stocks for Lactococcus lactis</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/b/b4/Uppsala2013_PreparationOfCEllsWesternblot.pdf">Preparation of cells Westernblot</a></li> | ||
| + | |||
| + | <li><a href="https://static.igem.org/mediawiki/2013/e/ec/Uppsala2013_Spectrophotometry_of_tyrosine_ammonia_lyase.pdf">Spectrophotometry of tyrosine ammonia lyase</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/7/7e/Uppsala2013_TBE-buffer_protocol.pdf">TBE-buffer protocol</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/8/81/Uppsala2013_Transformation_by_Electroporation_for_L.reuteri.pdf">Transformation by Electroporation for L.reuteri</a></li> | ||
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| + | <li><a href="https://static.igem.org/mediawiki/2013/5/59/Uppsala2013_Transforming_e-coli_protocol.pdf">Transforming e-coli protocol</a></li> | ||
| + | |||
| + | <li><a href="https://static.igem.org/mediawiki/2013/2/23/Uppsala2013_WesternBlot.pdf">WesternBlot</a></li> | ||
| + | |||
| + | </ul> | ||
</div> | </div> | ||
<div id="bottom-pic"> | <div id="bottom-pic"> | ||
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</div> | </div> | ||
Latest revision as of 20:02, 27 October 2013
Protocols
- 3A assembly cloning cycle
- Agarose gel electrophoresis
- Autoclaver manual
- BioBrick assembly manual
- CCMB80 buffer protocol
- Competent cell preparation
- Competitiontest
- High pressure liquid chromatography
- LB and LB-agar protocol
- M17 medium preparation, Lactococcus lactis
- Overnight culture and glycerol stock
- Phusion PCR Blank protocol
- Plasmidstability
- Preparation of stocks for Lactococcus lactis
- Preparation of cells Westernblot
- Spectrophotometry of tyrosine ammonia lyase
- TBE-buffer protocol
- Transformation by Electroporation for L.reuteri
- Transforming e-coli protocol
- WesternBlot
