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Team:Paris Saclay/Notebook/August/23
From 2013.igem.org
(Difference between revisions)
(→Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3) |
(→1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI) |
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* Well 1 : 6µL DNA Ladder | * Well 1 : 6µL DNA Ladder | ||
| - | * Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI+ | + | * Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI +1 µl of 6X loading dye |
* Gel : 1% | * Gel : 1% | ||
|} | |} | ||
Expect sizes : | Expect sizes : | ||
| - | * | + | * pSB3K3 : 2750 bp |
{| | {| | ||
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| - | We can see anything. The digestion | + | We can't see anything. The digestion didn't work. |
|} | |} | ||
Latest revision as of 01:21, 5 October 2013
Contents |
Notebook : August 23
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in pSB3K3
1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI
|
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Expect sizes :
- pSB3K3 : 2750 bp
|
We can't see anything. The digestion didn't work. |
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