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Team:Paris Saclay/Notebook/August/23
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(→1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI) |
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* Well 1 : 6µL DNA Ladder | * Well 1 : 6µL DNA Ladder | ||
| - | * Well 2 : 5µL of BBa_J04450 digested by EcoRI/ | + | * Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI +1 µl of 6X loading dye |
* Gel : 1% | * Gel : 1% | ||
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| - | We can see anything. The digestion | + | We can't see anything. The digestion didn't work. |
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Latest revision as of 01:21, 5 October 2013
Contents |
Notebook : August 23
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in pSB3K3
1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI
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Expect sizes :
- pSB3K3 : 2750 bp
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We can't see anything. The digestion didn't work. |
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