Latest revision as of 16:36, 4 October 2013

Notebook : July 10

Sheng

Abdou

Transformation of BBa_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations.

We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.

Abdou

@@ Line 1: / Line 1: @@
 {{Team:Paris_Saclay/incl_debut_generique}}
 ='''Notebook : July 10'''=
 =='''Lab work'''==
-constructing
+==='''A - Aerobic/Anaerobic regulation system'''===
+===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
+===='''1 - Transformation of BBa_I732019 in DH5α'''====
+Sheng
+Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
+===='''Objective : obtaining biobricks in pSB3K3'''====
+===='''1 - Mini and maxi preparation of the culture of BBa_J04450'''====
+Abdou
+{|
+| style="border:1px solid black;padding:5px;background-color:#DE;" |
+Transformation of BBa_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations.
+|}
+Protocol : [[Team:Paris_Saclay/mini_maxi|Mini and maxi preparation]]
+We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
+==='''B - PCB sensor system'''===
+===='''Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein'''====
+===='''1 - Transformation of ligation of pSB1C3 and BphA1 or BphR1 or BphR2'''====
+Abdou
+Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
-<br>
 {| border="1" align="center"
 |[[Team:Paris Saclay/Notebook/July/9|<big>Previous day</big>]]
-|[[Team:Paris_Saclay/Notebook|<big>Back to calender</big>]]
+|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
 |[[Team:Paris Saclay/Notebook/July/11|<big>Next day</big>]]