Team:Macquarie Australia/Protocols/SDS Page

From 2013.igem.org

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[[File:800px-Stain_for_SDS-PAGE_gel.jpg|500px|thumb|left|SDS-Page, image by Andra MIhali]]
<b>1)</b> Resuspend Pelleted cells in 200 uL Milli-Q H2O.
<b>1)</b> Resuspend Pelleted cells in 200 uL Milli-Q H2O.

Latest revision as of 06:29, 13 September 2013


SDS Page

SDS-Page, image by Andra MIhali

1) Resuspend Pelleted cells in 200 uL Milli-Q H2O.


2) Transfer 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer.


3) Using a Hamilton syringe, shear the cells.


4) Centrifuge the preparations for 3 mins @ 13,000 rpm.


5) Load 20 uL of the supernatant in to the gel.


6) Conduct electrophoresis at a constant voltage (200 V) for one hour.

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