Team:ETH Zurich/Experiments 7

From 2013.igem.org

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<h1>Beta-galactosidase(LacZ)</h1>
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<h1>Alkaline phosphatase(PhoA)</h1>
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<b>Chemical conversion</b><br>
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<h1>Acetyl esterase(Aes)</h1>
<h1>Acetyl esterase(Aes)</h1>
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<b>Chemical conversion</b><br>
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<h1>Beta-glucuronidase(GusA)</h1>
<h1>Beta-glucuronidase(GusA)</h1>
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<b>Chemical conversion</b><br>
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<h1>β-N-Acetylglucosaminidase(NagZ)</h1>
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<b>Chemical conversion</b><br>
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Revision as of 13:23, 13 September 2013

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Contents

What about those hydrolases ?

We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).

On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.

Beta-galactosidase(LacZ)

Chemical conversion
LacZhydro.png

Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.5: X-Gal on LacZ expressing colony
Fig.6: Beta-green-X-gal on LacZ expressing colony


Alkaline phosphatase(PhoA)

Chemical conversion
PhoAhydro.png

Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.2: NPP on PhoA expressing colony


Acetyl esterase(Aes)

Chemical conversion
Aeshydro.png

Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.1: Magenta butyrate on Aes expressing colony


Beta-glucuronidase(GusA)

Chemical conversion
GusAhydro.png

Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.3 Magenta glucuronide on GusA expressing colony


β-N-Acetylglucosaminidase(NagZ)

Chemical conversion
NagZhydro.png

Colorimetric response in liquid culture

Colorimetric response on LB-Agar