Team:Paris Saclay/Notebook/July/9

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(Difference between revisions)
(Notebook : July 9)
(Summary :)
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For regulation system :
For regulation system :
*extraction of BioBrick BBa_K1155000 in concentrated medium for further DNA sequencing.
*extraction of BioBrick BBa_K1155000 in concentrated medium for further DNA sequencing.
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For the construction of BioBrick
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*the terminator BBa_B0010 in PSB1A2 plasmid was transformed into competent cells and then was cultured on solid medium  
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*the terminator BBa_B0010 in PSB1A2 plasmid was transformed into competent cells and then was cultured on solid medium for confirmation
+
*the plasmid PSB3K3 was transformed into competent cells and then was cultured on solid medium for confirmation
*the plasmid PSB3K3 was transformed into competent cells and then was cultured on solid medium for confirmation
For sensor system:
For sensor system:

Revision as of 09:12, 21 September 2013

Notebook : July 9

Summary :

For regulation system :

  • extraction of BioBrick BBa_K1155000 in concentrated medium for further DNA sequencing.
  • the terminator BBa_B0010 in PSB1A2 plasmid was transformed into competent cells and then was cultured on solid medium
  • the plasmid PSB3K3 was transformed into competent cells and then was cultured on solid medium for confirmation

For sensor system:

  • A series of digestion, ligation were performed for BioBrick BphR2, BphR1, BphA1

Lab work

constructing



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