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Team:Paris Saclay/Notebook/July/9
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*extraction of BioBrick BBa_K1155000 in concentrated medium for further DNA sequencing. | *extraction of BioBrick BBa_K1155000 in concentrated medium for further DNA sequencing. | ||
*the terminator BBa_B0010 in PSB1A2 plasmid was transformed into competent cells and then was cultured on solid medium | *the terminator BBa_B0010 in PSB1A2 plasmid was transformed into competent cells and then was cultured on solid medium | ||
| - | *the plasmid PSB3K3 was transformed into competent cells and then was cultured on solid medium for confirmation | + | *the plasmid PSB3K3 was extracted form iGEM plate kit 2013 and transformed into competent cells and then was cultured on solid medium for confirmation |
For sensor system: | For sensor system: | ||
*A series of digestion, ligation were performed for BioBrick BphR2, BphR1, BphA1 | *A series of digestion, ligation were performed for BioBrick BphR2, BphR1, BphA1 | ||
Revision as of 12:07, 21 September 2013
Notebook : July 9
Summary :
For regulation system :
- extraction of BioBrick BBa_K1155000 in concentrated medium for further DNA sequencing.
- the terminator BBa_B0010 in PSB1A2 plasmid was transformed into competent cells and then was cultured on solid medium
- the plasmid PSB3K3 was extracted form iGEM plate kit 2013 and transformed into competent cells and then was cultured on solid medium for confirmation
For sensor system:
- A series of digestion, ligation were performed for BioBrick BphR2, BphR1, BphA1
Lab work
constructing
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