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Team:Paris Saclay/Notebook/July/10
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| - | Protocol : [[Team:Paris_Saclay/Protocols/ | + | Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]] |
===='''Objective : obtaining biobricks in PSB3K3'''==== | ===='''Objective : obtaining biobricks in PSB3K3'''==== | ||
Revision as of 18:25, 25 September 2013
Contents |
Notebook : July 10
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003, Bba_K1155007
1 - Transformation of Bba_I732019 and Bba_B0010 in DH5α
Sheng
|
Transformation of Bba_B0010 of 07/09/13 didn't work. We will do it again. |
Protocol : Bacterial transformation
Objective : obtaining biobricks in PSB3K3
1 - Mini and maxi preparation of the culture of Bba_J04450
Abdou
|
Transformation of Bba_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations. |
Protocol : Mini and maxi preparation
We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
B - PCB sensor system
Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2 protein
1 - Transformation of ligation of PSB1C3 and BphA1 or BphR1 or BphR2
Abdou
Protocol : Bacterial transformation
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