Team:Colombia Uniandes/How to parts
From 2013.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
{{Http://2013.igem.org/Team:Colombia Uniandes/Bootstrap}} | {{Http://2013.igem.org/Team:Colombia Uniandes/Bootstrap}} | ||
- | + | {| border="1" align="center" | |
+ | |+'''PCR reagents and amounts for one 50 ul reaction''' | ||
+ | | style="text-align: center;" |Name | ||
+ | | style="text-align: center;" |Sequence | ||
+ | | style="text-align: center;" |Identification Number | ||
+ | |||
+ | |- | ||
+ | | style="text-align: center;" |FW XbaI-BAP2 | ||
+ | | style="text-align: center;" |CACCTCTAGAGAGCATTAATGCAAGTCGAGAAAGG | ||
+ | | style="text-align: center;" |19 | ||
+ | |||
+ | |- | ||
+ | | style="text-align: center;" |Primer FW | ||
+ | | style="text-align: center;" |2,5 ul | ||
+ | |||
+ | |- | ||
+ | | style="text-align: center;" |Primer RV | ||
+ | | style="text-align: center;" |2,5 ul | ||
+ | |||
+ | |- | ||
+ | | style="text-align: center;" |DMSO | ||
+ | | style="text-align: center;" |2,0 ul | ||
+ | |- | ||
+ | | style="text-align: center;" |Master Mix | ||
+ | | style="text-align: center;" |25 ul | ||
+ | |- | ||
+ | | style="text-align: center;" |dH2O | ||
+ | | style="text-align: center;" |15 ul | ||
+ | |- | ||
+ | |} |
Revision as of 18:54, 25 September 2013
Name | Sequence | Identification Number |
FW XbaI-BAP2 | CACCTCTAGAGAGCATTAATGCAAGTCGAGAAAGG | 19 |
Primer FW | 2,5 ul | |
Primer RV | 2,5 ul | |
DMSO | 2,0 ul | |
Master Mix | 25 ul | |
dH2O | 15 ul |